Baking FINA Useless!!!

[[email protected]]

I put this as a reply in another thread but I wanted to see if I can get direct feedback on this.

How about filtering? What is the size of the filter? If it is small enough you may be keeping the bacteria out by filtering. They are .45u pore size.

Here is an excerpt from a web site:
To assess the microbiological population in water, one must trap existing micro-organisms on a .45u filter in a specified volume of water. The filter is then placed directly on nutrient agar. This will render a numerical value of colony forming units (CFU) per volume of water. Identification of isolated colonies on the membrane filter can then proceed by standard microbiological methods.

It looks like a .45u syringe filter will trap most bacteria.

 

[Paiddailey]

Noted,
Thanks for the info...
Peace

 

[Fonz]

I put this as a reply in another thread but I wanted to see if I can get direct feedback on this.

How about filtering? What is the size of the filter? If it is small enough you may be keeping the bacteria out by filtering. They are .45u pore size.

Here is an excerpt from a web site:
To assess the microbiological population in water, one must trap existing micro-organisms on a .45u filter in a specified volume of water. The filter is then placed directly on nutrient agar. This will render a numerical value of colony forming units (CFU) per volume of water. Identification of isolated colonies on the membrane filter can then proceed by standard microbiological methods.

It looks like a .45u syringe filter will trap most bacteria.

If I were you, I'd check in the Medical library how many kind sof bacteria can fit through the .22 - .45 micron difference in filters.

A whole hell of a lot.

Fonz

 

[[email protected]]

Between alcohol and the filter not much. Since even bacteria can survive 250 degrees I think the mail concern would be to minimize bacteria. It becomes a decision of what steps are necessary and acceptable to the individual.

Alcohol, cooking, and filtering will still leave some bacteria in there. We can toss UV and some other methods in there too. If you are degrading the product you have to think about what is “reasonable”.

The small amount of bacteria left in the bottle after the process can also start to reproduce. Since you have killed the weaker strains the stronger ones will now breed (strong) bacteria. Some are naturally resistant to a harsh environment.

 

[Sigmund Roid]

Autoclaving will kill ALL bacteria. Put your liquid in a pressure resistive vial, close it very well and then put it in an autoclaving machine or a pressure cooking pan, and expose to steam and a temperature of 120 C for at least 15 minutes. Your fina will be fine, as long as there is no contact with oxygen or water.

 

[[email protected]]

To sterilize 100% you'll need a 0.22µm pore size filter. That;s more for water base. Oil will prob rip the shit out of it.

Autoclaving? Not something you can pick up at your corner store!?!

 

[[email protected]]

If done correctly autoclaving is the best and most dependable method of sterilization.

The heat damages all cellular macromolecular structures including nucleic acids and the cytoplasmic membrane.

Steam sterilization employs steam under (15 psi) pressure to attain a temperature of 121 C which is necessary to kill endospores. Moist heat is more effective and rapid than dry in part due to the better heat transfer and penetration to all areas of the load during a sterilization cycle. (10 - 15 l need ONE HOUR of heating to get to 121 PLUS an extra 15 min of sterilization time - Volume of load important)

Using an oven to provide dry heat to sterilize objects requires higher temperatures and much longer exposure times (160 C for at least 2 hours).


OK I am dizzy!

 

[anabolic24/7]

From personal experiance, I baked my first batch years ago, and have never done so since.
I dont even remember how many kits I've made now for myself and others, all unbaked and never a problem.
I am careful to use a clean enviroment, and not let needles ect touch anything.

 

[Sigmund Roid]

To sterilize 100% you'll need a 0.22µm pore size filter. That;s more for water base. Oil will prob rip the shit out of it.

Autoclaving? Not something you can pick up at your corner store!?!

Yes, you can, the device is called a pressure cooking pan for the kitchen cooks. Works like a charm. Just make sure your vials and stoppers or lids or whatever can stand the high temperature and pressure.