The Myth Of "Keepable" Gains

[Wrongun]

Nelson i would agree especially with the analogy with the ''pump''

Training and med methodology has changed a lot over the years (not always for the better IMHO)

I see dosages have increased 10 fold but there again i see so many rely on PCT to keep the gains. A lot IMHO is in physchological as well and pre PCT knowledge i remember i used lots of ephedrine to keep the intensity up and also taper all dosages.

This is against many modern day thinking with PCT BUT when i mixed the both PCT and the high intensity training approach i found gains very keepable

Good thread this

Wrongun!

 

[Magick69]

Well, trib has been around long enough for everyone to know how effective it is, and I'm not impressed. I've used every kind imaginable. I use to get Tribex for free when I worked for T-mag and took bottles of the stuff -- up to 6X's the recommended dose -- and NOTHING.

Tongkat Ali looks good on paper but it doesn't have the "kick" of either muara puama or avenacosides IMO.

Plus, I've conducted tests with the use of avenacosides and free test and the results were undeniable. It works. It varies from individual to individual but everyone's numbers went up.

WRONGUN: Good question. I think PCT can help with keepable gains because it prevents the catabolic state that occurs post cycle -- but it's a small part of the equation. Proper PCT prevent problems and helps recovery but muscle mass is maintained more through necessity. The body needs a reason to hold the muscle. That means continued training PLUS the ability to recover. One way is through sleep and nutrition and the other is through having a high enough free testosterone level to support the muscle. If you then stay in an anabolic state the muscle becomes "solidified" because the body is used to holding it. Once again, it comes down to not having a dependance.

The issue of blood volume is very overlooked. The "pump" has been neglected in favor of more HIT style training but the pump is a HUGE factor in maintaining muscle size and shape. If blood volume is kept high, gains are easier to keep.

The way I've always viewed steroid use for keepable gains is to use them to get to a place that is beyond natural training, then holding it. If you gain too much, or stay on too long, or use dosages that are too high, you essentially are making it MORE differicult to maintain gains. But all in all, yes, guys who've used steroids will tend to carry more muscle after being natural if they know what they're dong.

sorry bro but still there is the problem of studies which show increase in testo

LJ100 definitevely has few of them and still other underway

Tribulus has few of them although some controversial

muira puama???? there is not only one study to show increase in testosterone so why bother??

 

[boston789]

LJ100 is supposed to be awesome. I might try some....

Anyone have any experience with this stuff?

 

[no1_brawler]

I keep seeing these articles and it puts steroids down. After reading something like this, it makes a person think steroids dont do much. And ive said this many times, my first cycle was last year when i was 18yrs old and i gained 35lbs in a 11 week Test E cycle 600mg/wk. I kept 33lbs. Now this is with minimal fat gain, i still kept my six pack. I gained back the 2lbs after 3 weeks of PCT. Thats a 35lb gain, explain that. Also i got stronger during PCT, explain that. Steroids are just amazing, not sure how it effects everyone else but my first time was crazy.

 

[chazk]

I keep seeing these articles and it puts steroids down. After reading something like this, it makes a person think steroids dont do much. And ive said this many times, my first cycle was last year when i was 18yrs old and i gained 35lbs in a 11 week Test E cycle 600mg/wk. I kept 33lbs. Now this is with minimal fat gain, i still kept my six pack. I gained back the 2lbs after 3 weeks of PCT. Thats a 35lb gain, explain that. Also i got stronger during PCT, explain that. Steroids are just amazing, not sure how it effects everyone else but my first time was crazy.

Now stop taking steroids for 2-3 years keep a journal and get back with us.

you also mentioned you were 18 chances are you prob would have gained 20lbs just by growing ,eating correct and not even using steroids to begin with...

hell i went from 135lbs to 150lbs in my first year just playing foot ball with out any roids at all from age 16-17 not even lifting then from 17-18 got up to 175 a gain of 25lbs by lifting and eating better but for the most part it was called becoming a adult just normal growth bone mass ,got taller,shoulders filled out it was called the end stages of puberty.

get it now?

hey your only 18 so i'll be eazy on ya. Most of it was gains you wil keep becuase you were gonna fill out anyways building bone mass ,height ,just becoming a adult in general

were talking about guys that have pushed the envalope done lots of cycles and relize after a set point its hard to maintain the drug induced muscle ..
Not newbies 18 gaining making gains while finishing puberty when most of it was just growing up to become a adult.

 

[no1_brawler]

Now stop taking steroids for 2-3 years keep a journal and get back with us.

you also mentioned you were 18 chances are you prob would have gained 20lbs just by growing ,eating correct and not even using steroids to begin with...

hell i went from 135lbs to 150lbs in my first year just playing foot ball with out any roids at all from age 16-17 not even lifting then from 17-18 got up to 175 a gain of 25lbs by lifting and eating better but for the most part it was called becoming a adult just normal growth bone mass ,got taller,shoulders filled out it was called the end stages of puberty.

get it now?

hey your only 18 so i'll be eazy on ya. Most of it was gains you wil keep becuase you were gonna fill out anyways building bone mass ,height ,just becoming a adult in general

were talking about guys that have pushed the envalope done lots of cycles and relize after a set point its hard to maintain the drug induced muscle ..
Not newbies 18 gaining making gains while finishing puberty when most of it was just growing up to become a adult.

It was a 11 week cycle, i dont think i was growing taller with bigger bones in that time frame. And you said you gained 25lbs in one year, i gaiend 35lbs in 11 weeks, As you can see, much much faster. And stop making it out to be that i woudlve gained the weight naturally. I was eating 3500calories a day and doing all the big compound movements and i wouldnt gain weight for a good 6months or so. While taking the Test E, i was eating the same 3500cals and workin out a bit harder coz i was on gear. So dont make it sound like i coulve gained that much naturally.

 

[Nelson Montana]

sorry bro but still there is the problem of studies which show increase in testo

LJ100 definitevely has few of them and still other underway

Tribulus has few of them although some controversial

muira puama???? there is not only one study to show increase in testosterone so why bother??

I may be presumptuos it but sounds like you're trying to push a product here.

I was onto LJ years ago because it looked so good on paper. We did tests with LJ and Trib and they didn't stand up to avenacosides and muara puama. As far as studies with Muara Puama , there haven't been many but there there have been a few that showed very positive results. (Couldn't find them on-line though I'll track then down if you feel like finding them). And the empirical evidence of long term use as a libido booster is pretty unanamous. Still. it's more the avenacosides A&B that have the major effect on FREE testosterone.

There isn't a single study on tribulus that shows it as being effective that I find credable -- which would be fine if I felt anything from it. Some do. I don't. I can't factor what might be placebo into a formula I design. I had to go with what I knew worked and what my studies concluded.

no1 -- chazk is right. At your age you "held" on to gains because you are naturally in a growth stage at that point in your life. No experienced bodybuilder gains 35 lbs of muscle from gear in 11 weeks and keeps it.

 

[Ross]

Preliminary Biological Activity:
Preliminary biological activity:
i) testosterone level
- incubation of E. longifolia aqueous extract in rat testicular homogenate
- steroid hormones (testosterone) analysis – capillary gas chromatography
Steroid Hormone Level LJ100 ®Extract Control
Testosterone 3.91 ±0.73 1.53 ±0.19
Progesterone 23.62 ±1.25 trace
17-OH Progesterone 5.28 ±0.46 0.95 ±0.23

LJ100® helps to activate enzymes activities that convert pregnenolone and 17-OH pregnenolone into progesterone and 17-OH progesterone.


Control
Prenenolone
LJ100®

An a
2.52
1.62
6.64

*
+0.12
+0.28
+0.44

An b &
2.64
1.99
0.38

Andien b
+0.09
+0.44
+0.11

An a, an b, and andien b are steroid metabolites that belongs to 16-androstenes steroid family, also known as pheromones are axillary secretion responsible in the synthesis of odour. An a plays an important role in communication, psychological and sexual behavior both in human and animals. This study shows that LJ100® is not only capable in increasing the testosterone production but at the same time it also influences the synthesis of pheromones.


Pregnenolone metabolite analysis in mice
ug steroid/10 mg protein

Steroid Extract Blank Control LJ100® (2) LJ100 (3)
5a-androstenone * * * *
androstenedione * * * 0.08
andien b & an-b 4.05 2.68 0.88 1.42
an-a 22.57 32.7 109.99 207.26
5-androstediol * * * *
5a-DHT * * * *
4-androstenedione * * * 0.07
testosterone 0.98 1.68 2.43 2.87
5a-androstane-diol * * * *
7-OH preg 2.43 5.15 1.41 1.31
progesterone 3.36 6.39 12.30 13.20

Note that there is no elevation of the dihydrotestosterone.


ug steroid/10 mg protein

Steroid % increase Blank Control LJ100® (2) LJ100® (3)
testosterone 180% 0.98 1.68 2.43 2.87
progesterone 190% 3.36 6.39 12.30 13.20

Steroid metabolite analysis (in human testes)

ug steroid/10 mg protein

Steroid Derivatives Control Pregnenolone LJ100®
5a-Androstane-3a,17 b-diol 5.12± 1.06 7.89 ± 0.82 5.75 ±0.32
5-Androstenediol 8.19 ±0.31 11.39 ±0.75 7.42 ±0.19
5a-Dihydrotestosterone 21.24 ±2.13 25.44 ±2.25 20.53 ±0.61
16-Dehydropregnenolone 3.72 ±2.04 3.94 ±0.91 4.86 ±0.94
testosterone 2.48 ±0.96 2.91 ±0.76 12.91 ±1.0
17-Hydroxypregnenolone 0.11 ±0.02 0.76 ±0.04 0.09 ±0.00
4-Androstenedione 18.33 ±4.21 21.58 ±0.94 24.51 ±1.83

Treatment effect towards testosterone concentration in rat Leydig Cells

Treatment Testosterone Concentration (pg/ml) % increase
Control 8.92 ±1.68
hCG 13.14 ±2.61 47.27
lac 13.03 ±3.10 46.11
LJ100® 19.38 ±2.70 119.77

hCG- Human Chrorionic Gonadotropin

lac-lactate

Based on the steroid biosynthesis pathways, CYP17 was selected for this study;

CYP17 that converts pregnelolone ® 17-OH pregnenolone ® DHEA or Progesterone ® 17-OH progesterone ® Androstenedione

Relative values for CYP17 gene following incubation with LJ100®

Treatment
Relative Values

hcg
1.157 ± 282.0

LJ100®
3.807 ± 0.590


CYP17 (17 a-hyroxylase/17,20 lyase) involves in the early stage of steroid biosynthesis. Result from this study showed that LJ100 ®significantly increased the expression of CYP17 gene compred to the positive control (hCG). The observed effect towards CYP17 gene expression might suggests that more of this enzyme is being produced, which will enhanced the matabolism of pregnenolone and 17-OH pregnenolone to yield more dehyroepiandrosterone (DHEA) as well as the metabolism of progesterone and 17-OH progesterone to 4-androstenedione. This process is important in testosterone biosynthesis as DHEA and 4-androstenedione will be directly converted to testosterone.




--------------------------------------------------------------------------------

Anabolic Study of LJ100®
Sareena Hanim Hamzah & Ashril Yusof

Department of Exercise Physiology, Sports Centre, University of Malaya, Kuala Lumpur

Testosterone is renown biochemically for its anabolic nature and net effect of increasing metabolic rate and enhancing the process of biosynthesis. In this study, seven male subjects aged between 26-32 years took 100mg of LJ100 for a period of 8 weeks. Simultaneously, subjects performed an intensive strength training program with initial load of 60% RM, which was carried out on alternate days. Measurement of skin fold thickness, arm circumference, one repetition maximum (1RM) strength on the upper limb and the electormyographic activity of biceps were recorded before and after the period of consumption of LJ100®.

A double blind study on 7 controls was conducted (100 mg lactose) simultaneously. Fat free mass increased from 52.3 (7.1) to 54.4 (7.4) kg (p<0.05). The percentage of fat decreased significantly from 31.3 (5.5) to 28.4 (6.4) % (p<0.05). The arm circumferences of the participants were observed to increase from 30.9 (1.9) to 32.7 (2.0) cm (p < 0.05). The 1RM muscle strength test showed an increment from 73.7 (16.6) to 78.7 (17.0) kg (p < 0.05). The mean frequency of sEMG on bicep muscle contraction of the subjects showed a more significant improvement in strength (p<0.05) compared with the controls (p>0.05). This shows that when Eurycoma administered together with exercise gave a greater gain in strength. The results suggest that LJ100® standardized extract of Eurycoma longifolia Jack can have an anabolic effect on muscle cells.

Results:

Placebo (100mg/day) LJ100® Eurycoma Extract (100mg/day)
Parameters Pre (mean + SD) Post (mean ± SD)
Pre (mean + SD) Post (mean ± SD)
FFM (kg) 52.44 + 3.77 52.77 + 7.18 52.26 + 7.18 54.39 + 7.43*
Fat Mass (%) 22.83 + 2.43 21.33 + 2.35* 31.30 + 5.48 28.44 + 6.43*
1 RM 77.29 + 8.90 79.43 + 8.83 73.71 + 8.90 78.71 + 17.00*
Arm Cir (cm) 29.8 + 3.70 30.7 + 3.86 30.87 + 1.88 32.67 + 1.96*
sEMG (mV) 127.95 + 30.90 98.8 + 50.1 121.77 + 40.0 90.47 + 64.6*

*Results of mean ± SD for pre and post experiment showed significant difference (p<0.05)

The mean frequency of sEMG on bicep muscle contraction f the subjects showed a more significant improvement in strength (p<0.05) compared with the controls (p>0.05). The results suggest that LJ100® has an anabolic effect on muscle cells.


--------------------------------------------------------------------------------

LJ100® effects on Total Testosterone, DHEA, & SHBG

University of Malaysia, Dr. Ismail Tambe

Conclusions:

Total testosterone levels do not show LJ100® does not disrupt steroidogensis. This suggests the feedback system is activated to ensure the testosterone levels are within the individuals needs range. (This confirms that LJ100® is not a steroid that will result in the unhealthy side effects of steroidal use.)

Analysis of DHEA showed gradual increase in the level from 26% after 1 week to 47% after 3 weeks (using 100mg dose). This suggests that the extract may influence the DHEA production, which would in turn subsequently be aromatized to testosterone.

SHBG (Sex Hormone Binding Globulan) analysis showed that levels were reduced in 36% of the cases after one week. The reduction went up to 66% after 3 weeks. This suggests that the extract could have an effect on the production of SHBG. (Reduction in SHBG indicates less protein to bind with androgen and therefore more free androgen for use by organs. A reduction will also reduce fat production.)


--------------------------------------------------------------------------------

Saliva Testosterone Test of 9 Individuals 26-52 years of Age

¢Dosage 2x2(50mg/capsules) morning & evening for 10 days

¢Normal range for athlete 800 = 150ng/dl of blood



Volunteer age pre treatment after treatment %

ng/dl blood ng/dl blood Increase

1 26 860 = 30 1,650 = 50 91.86%

2 28 580 = 30 985 = 35 69.83%

3 35 875 = 40 1, 576 = 60 80.11%

4 24 950 = 45 2,210 = 55 132.63%

5 29 755 = 30 1,345 = 35 78.15%



6 48 650 = 20 875 = 30 34.62%

7 52 450 = 25 765 = 35 70.00%

8 50 585 = 25 875 = 35 49.57%

9 42 350 = 30 480 = 35 37.14%



Data – preliminary data - more work to be carried out

Volunteers 1-5 are athletes - data are an average of 3 different studies at different times

Volunteers 6-9 do not exercise on a regular basis




--------------------------------------------------------------------------------

Effects of E. longifolia on animal testosterone


Animal
Increase %

Mice
479%

Rat
380%

Rabbit
320%

*Human testicular homogenate ( in vitro)


440%


J.M Saad et al 1995






--------------------------------------------------------------------------------



Effect of LJ100® E.longifolia extract on the levels of cAMP and cGMP of rabbit corpus cavernosa.

In this study, the mechanism of action of LJ100® Eurycoma longifolia extract on penile erection was assessed by determining the in vitro formation of cGMP and cAMP in rabbit corpus carvenosum. The effect of E. longifolia was then compared with the effectiveness of sildenafil citrate (viagra) in triggering penile erection. Corpus cavernosum tissues were treated with LJ100® E.longifolia extract and sildenafil citrate at different concentrations and incubation time. This was done by incubating the rabbit tissues in Dulbelco’s Minimum Essential Medium (MEM) containing various concentration of extract (0, 1.25, 1.875, 2.5, 3.125 and 3.75 µg/ml) and then measuring the cGMP and cAMP level using an enzyme-linked immunoassay (EIA) kit. Prior to this, the optimum concentration of sodium nitroprusside (SNP) as a stimulus for nitric oxide formation and guanylate cyclase activated, were determined.

Significant findings


1. LJ100® E.longifolia extract increased the level of cGMP in rabbit corpus cavernosum to almost 4-fold.



In the presence of SNP (10 µm) LJ100® E. longifolia extract increased cGMP in rabbit corpus cavernosum with increasing concentrations (1.25 – 3.125 µg/ml). The effective concentration of LJ100® E.longifolia extract is 3.125 µg/ml at 30 minutes incubation. The increase was greatest (5.298pM/mg tissue) compared to control (1.2pM/mg tissue), representing a 4-fold increase. For comparison, a similar study was also carried out using sildenafil citrate, an anti-impotency pill known to act via elevation of cGMP. Sildenafil citrate increases cGMP in rabbit corpus cavernosum with increasing concentrations (10-7-10-4M) in response to SNP (10µM). The effective concentration of sildenafil citrate is 10-4M which increases cGMP up 4.832pM/mg tissue compared to control (0.8pM/mg tissue). The erectogenic effect of sildenafil citrate is mediated by specific enhancement of cGMP accumulation in the corpus cavernosum, consistent with the known activity of sildenafil as a potent and selective inhibitor of cGMP-phosphodiesterase 5 (cGMP-PDE5). The result from preliminary study shows that the mechanism of action of LJ100® E.longifolia extract is similar to sildenafil citrate.



2. LJ100® E.longifolia extract enhances the level of cAMP in rabbit corpus cavernosum; a phenomenon not observed with Viagra.



Effect of sildenafil citrate with concenration 10-4M, 10-5M, 10-6M and 10-7M on cAMP levels also was studied in this research. From the results, LJ100® E.longifolia extract was found to increase cAMP levels in corpus cavernosum and there were no significant increases of cAMP levels in the corpus cavernosum tissue treated with Viagra.



Results of this study validate the physiological observations of the aphrodisiac properties of E.longifolia whereby LJ100® E.longifolia extract is found to increase and enhance the levels of cGMP and cAMP on a time and concentration dependent manner in the rabbit corpus cavernosa tissue, even in the absence of sexual stimuli. The increase in both second messengers indicates smooth muscle relaxation and this can be extrapolated to a penile erection in a in vivo.

 

[Nelson Montana]

Preliminary Biological Activity:
Preliminary biological activity:
i) testosterone level
- incubation of E. longifolia aqueous extract in rat testicular homogenate
- steroid hormones (testosterone) analysis – capillary gas chromatography
Steroid Hormone Level LJ100 ®Extract Control
Testosterone 3.91 ±0.73 1.53 ±0.19
Progesterone 23.62 ±1.25 trace
17-OH Progesterone 5.28 ±0.46 0.95 ±0.23

LJ100® helps to activate enzymes activities that convert pregnenolone and 17-OH pregnenolone into progesterone and 17-OH progesterone.


Control
Prenenolone
LJ100®

An a
2.52
1.62
6.64

*
+0.12
+0.28
+0.44

An b &
2.64
1.99
0.38

Andien b
+0.09
+0.44
+0.11

An a, an b, and andien b are steroid metabolites that belongs to 16-androstenes steroid family, also known as pheromones are axillary secretion responsible in the synthesis of odour. An a plays an important role in communication, psychological and sexual behavior both in human and animals. This study shows that LJ100® is not only capable in increasing the testosterone production but at the same time it also influences the synthesis of pheromones.


Pregnenolone metabolite analysis in mice
ug steroid/10 mg protein

Steroid Extract Blank Control LJ100® (2) LJ100 (3)
5a-androstenone * * * *
androstenedione * * * 0.08
andien b & an-b 4.05 2.68 0.88 1.42
an-a 22.57 32.7 109.99 207.26
5-androstediol * * * *
5a-DHT * * * *
4-androstenedione * * * 0.07
testosterone 0.98 1.68 2.43 2.87
5a-androstane-diol * * * *
7-OH preg 2.43 5.15 1.41 1.31
progesterone 3.36 6.39 12.30 13.20

Note that there is no elevation of the dihydrotestosterone.


ug steroid/10 mg protein

Steroid % increase Blank Control LJ100® (2) LJ100® (3)
testosterone 180% 0.98 1.68 2.43 2.87
progesterone 190% 3.36 6.39 12.30 13.20

Steroid metabolite analysis (in human testes)

ug steroid/10 mg protein

Steroid Derivatives Control Pregnenolone LJ100®
5a-Androstane-3a,17 b-diol 5.12± 1.06 7.89 ± 0.82 5.75 ±0.32
5-Androstenediol 8.19 ±0.31 11.39 ±0.75 7.42 ±0.19
5a-Dihydrotestosterone 21.24 ±2.13 25.44 ±2.25 20.53 ±0.61
16-Dehydropregnenolone 3.72 ±2.04 3.94 ±0.91 4.86 ±0.94
testosterone 2.48 ±0.96 2.91 ±0.76 12.91 ±1.0
17-Hydroxypregnenolone 0.11 ±0.02 0.76 ±0.04 0.09 ±0.00
4-Androstenedione 18.33 ±4.21 21.58 ±0.94 24.51 ±1.83

Treatment effect towards testosterone concentration in rat Leydig Cells

Treatment Testosterone Concentration (pg/ml) % increase
Control 8.92 ±1.68
hCG 13.14 ±2.61 47.27
lac 13.03 ±3.10 46.11
LJ100® 19.38 ±2.70 119.77

hCG- Human Chrorionic Gonadotropin

lac-lactate

Based on the steroid biosynthesis pathways, CYP17 was selected for this study;

CYP17 that converts pregnelolone ® 17-OH pregnenolone ® DHEA or Progesterone ® 17-OH progesterone ® Androstenedione

Relative values for CYP17 gene following incubation with LJ100®

Treatment
Relative Values

hcg
1.157 ± 282.0

LJ100®
3.807 ± 0.590


CYP17 (17 a-hyroxylase/17,20 lyase) involves in the early stage of steroid biosynthesis. Result from this study showed that LJ100 ®significantly increased the expression of CYP17 gene compred to the positive control (hCG). The observed effect towards CYP17 gene expression might suggests that more of this enzyme is being produced, which will enhanced the matabolism of pregnenolone and 17-OH pregnenolone to yield more dehyroepiandrosterone (DHEA) as well as the metabolism of progesterone and 17-OH progesterone to 4-androstenedione. This process is important in testosterone biosynthesis as DHEA and 4-androstenedione will be directly converted to testosterone.




--------------------------------------------------------------------------------

Anabolic Study of LJ100®
Sareena Hanim Hamzah & Ashril Yusof

Department of Exercise Physiology, Sports Centre, University of Malaya, Kuala Lumpur

Testosterone is renown biochemically for its anabolic nature and net effect of increasing metabolic rate and enhancing the process of biosynthesis. In this study, seven male subjects aged between 26-32 years took 100mg of LJ100 for a period of 8 weeks. Simultaneously, subjects performed an intensive strength training program with initial load of 60% RM, which was carried out on alternate days. Measurement of skin fold thickness, arm circumference, one repetition maximum (1RM) strength on the upper limb and the electormyographic activity of biceps were recorded before and after the period of consumption of LJ100®.

A double blind study on 7 controls was conducted (100 mg lactose) simultaneously. Fat free mass increased from 52.3 (7.1) to 54.4 (7.4) kg (p<0.05). The percentage of fat decreased significantly from 31.3 (5.5) to 28.4 (6.4) % (p<0.05). The arm circumferences of the participants were observed to increase from 30.9 (1.9) to 32.7 (2.0) cm (p < 0.05). The 1RM muscle strength test showed an increment from 73.7 (16.6) to 78.7 (17.0) kg (p < 0.05). The mean frequency of sEMG on bicep muscle contraction of the subjects showed a more significant improvement in strength (p<0.05) compared with the controls (p>0.05). This shows that when Eurycoma administered together with exercise gave a greater gain in strength. The results suggest that LJ100® standardized extract of Eurycoma longifolia Jack can have an anabolic effect on muscle cells.

Results:

Placebo (100mg/day) LJ100® Eurycoma Extract (100mg/day)
Parameters Pre (mean + SD) Post (mean ± SD)
Pre (mean + SD) Post (mean ± SD)
FFM (kg) 52.44 + 3.77 52.77 + 7.18 52.26 + 7.18 54.39 + 7.43*
Fat Mass (%) 22.83 + 2.43 21.33 + 2.35* 31.30 + 5.48 28.44 + 6.43*
1 RM 77.29 + 8.90 79.43 + 8.83 73.71 + 8.90 78.71 + 17.00*
Arm Cir (cm) 29.8 + 3.70 30.7 + 3.86 30.87 + 1.88 32.67 + 1.96*
sEMG (mV) 127.95 + 30.90 98.8 + 50.1 121.77 + 40.0 90.47 + 64.6*

*Results of mean ± SD for pre and post experiment showed significant difference (p<0.05)

The mean frequency of sEMG on bicep muscle contraction f the subjects showed a more significant improvement in strength (p<0.05) compared with the controls (p>0.05). The results suggest that LJ100® has an anabolic effect on muscle cells.


--------------------------------------------------------------------------------

LJ100® effects on Total Testosterone, DHEA, & SHBG

University of Malaysia, Dr. Ismail Tambe

Conclusions:

Total testosterone levels do not show LJ100® does not disrupt steroidogensis. This suggests the feedback system is activated to ensure the testosterone levels are within the individuals needs range. (This confirms that LJ100® is not a steroid that will result in the unhealthy side effects of steroidal use.)

Analysis of DHEA showed gradual increase in the level from 26% after 1 week to 47% after 3 weeks (using 100mg dose). This suggests that the extract may influence the DHEA production, which would in turn subsequently be aromatized to testosterone.

SHBG (Sex Hormone Binding Globulan) analysis showed that levels were reduced in 36% of the cases after one week. The reduction went up to 66% after 3 weeks. This suggests that the extract could have an effect on the production of SHBG. (Reduction in SHBG indicates less protein to bind with androgen and therefore more free androgen for use by organs. A reduction will also reduce fat production.)


--------------------------------------------------------------------------------

Saliva Testosterone Test of 9 Individuals 26-52 years of Age

¢Dosage 2x2(50mg/capsules) morning & evening for 10 days

¢Normal range for athlete 800 = 150ng/dl of blood



Volunteer age pre treatment after treatment %

ng/dl blood ng/dl blood Increase

1 26 860 = 30 1,650 = 50 91.86%

2 28 580 = 30 985 = 35 69.83%

3 35 875 = 40 1, 576 = 60 80.11%

4 24 950 = 45 2,210 = 55 132.63%

5 29 755 = 30 1,345 = 35 78.15%



6 48 650 = 20 875 = 30 34.62%

7 52 450 = 25 765 = 35 70.00%

8 50 585 = 25 875 = 35 49.57%

9 42 350 = 30 480 = 35 37.14%



Data – preliminary data - more work to be carried out

Volunteers 1-5 are athletes - data are an average of 3 different studies at different times

Volunteers 6-9 do not exercise on a regular basis




--------------------------------------------------------------------------------

Effects of E. longifolia on animal testosterone


Animal
Increase %

Mice
479%

Rat
380%

Rabbit
320%

*Human testicular homogenate ( in vitro)


440%


J.M Saad et al 1995






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Effect of LJ100® E.longifolia extract on the levels of cAMP and cGMP of rabbit corpus cavernosa.

In this study, the mechanism of action of LJ100® Eurycoma longifolia extract on penile erection was assessed by determining the in vitro formation of cGMP and cAMP in rabbit corpus carvenosum. The effect of E. longifolia was then compared with the effectiveness of sildenafil citrate (viagra) in triggering penile erection. Corpus cavernosum tissues were treated with LJ100® E.longifolia extract and sildenafil citrate at different concentrations and incubation time. This was done by incubating the rabbit tissues in Dulbelco’s Minimum Essential Medium (MEM) containing various concentration of extract (0, 1.25, 1.875, 2.5, 3.125 and 3.75 µg/ml) and then measuring the cGMP and cAMP level using an enzyme-linked immunoassay (EIA) kit. Prior to this, the optimum concentration of sodium nitroprusside (SNP) as a stimulus for nitric oxide formation and guanylate cyclase activated, were determined.

Significant findings


1. LJ100® E.longifolia extract increased the level of cGMP in rabbit corpus cavernosum to almost 4-fold.



In the presence of SNP (10 µm) LJ100® E. longifolia extract increased cGMP in rabbit corpus cavernosum with increasing concentrations (1.25 – 3.125 µg/ml). The effective concentration of LJ100® E.longifolia extract is 3.125 µg/ml at 30 minutes incubation. The increase was greatest (5.298pM/mg tissue) compared to control (1.2pM/mg tissue), representing a 4-fold increase. For comparison, a similar study was also carried out using sildenafil citrate, an anti-impotency pill known to act via elevation of cGMP. Sildenafil citrate increases cGMP in rabbit corpus cavernosum with increasing concentrations (10-7-10-4M) in response to SNP (10µM). The effective concentration of sildenafil citrate is 10-4M which increases cGMP up 4.832pM/mg tissue compared to control (0.8pM/mg tissue). The erectogenic effect of sildenafil citrate is mediated by specific enhancement of cGMP accumulation in the corpus cavernosum, consistent with the known activity of sildenafil as a potent and selective inhibitor of cGMP-phosphodiesterase 5 (cGMP-PDE5). The result from preliminary study shows that the mechanism of action of LJ100® E.longifolia extract is similar to sildenafil citrate.



2. LJ100® E.longifolia extract enhances the level of cAMP in rabbit corpus cavernosum; a phenomenon not observed with Viagra.



Effect of sildenafil citrate with concenration 10-4M, 10-5M, 10-6M and 10-7M on cAMP levels also was studied in this research. From the results, LJ100® E.longifolia extract was found to increase cAMP levels in corpus cavernosum and there were no significant increases of cAMP levels in the corpus cavernosum tissue treated with Viagra.



Results of this study validate the physiological observations of the aphrodisiac properties of E.longifolia whereby LJ100® E.longifolia extract is found to increase and enhance the levels of cGMP and cAMP on a time and concentration dependent manner in the rabbit corpus cavernosa tissue, even in the absence of sexual stimuli. The increase in both second messengers indicates smooth muscle relaxation and this can be extrapolated to a penile erection in a in vivo.

What this shows me is that LJ increases progesterone. Maybe that's why it had no effect on me or anyone else I know who's tried it.

 

[no1_brawler]

I didnt know you were referring to bodybuilders. I believe i gained the 35lbs and kept it is because i was far from my genetic limit and it was my first cycle