Jinotropin GH: Cadaver Salvage or Manufactured?

[gorilla_boy]

Trust me bro, its a source post....no further explanation needed.

Well, if you guys hadn't been so damn secretive the legitimacy of this company and it's product could have been established many typed letters ago...

http://www.gensci.com.cn/green/english/products.htm

 

[gorilla_boy]

Please feel free to use the karma system.

 

[Hardcoregrowth]

I hit you up with some green gorilla_boy!

 

[Dr. M]

If you actually know what you're talking about then please describe to me (in layman's terms if possible) the process for removing GH from a pituitary gland and whatever equipment it takes to make it usable contrasting it against the process and equipment required to synthesize rHGH.

I'm betting that taking it from the pituitary gland is easier.

Gorilla_boy, I do this sort of shit for a living. I know what I'm talking about. Don't call me a defensive asshole when you're the one taking offence at a small, calm statement. Bullshit like this human pituitary extraction you're promoting is how rumours get started and live on as urban legends you tell your kids: "don't use GH - it's from dead bodies' pituitaries!"

From the pituitary:
-obtain cadaver tissue and remove relevant gland.
-homogenize tissue and prepare crude soluble extract (hard spin to pellet extraneous DNA, cell membrane, organelles, etc.)
-from soluble extract, perform crude salting out (ammonium sulfate cuts) and localize fraction which contains maximal hGH concentration. Lyophilize (freeze-dry) to minimize volume if desired.

From this point on you can take a number of steps, but the most likely ones would be applying a rehydrated (smaller volume) lyophilized sample to some sort of affinity column for a rough purification. Failing that, large-scale preparative HPLC could be run - the equipment for this alone would total millions for a commercial operation. There are many methods for preparation, and the process would almost certainly require a number of steps. You're trying to remove a single, low-concentration protein in MILLIGRAM amounts (for protein work, this is a TON) from a proteome of hundreds of thousands of proteins and millions of degradation products and other contaminating chemicals found within mammalian cells.

You're looking at a recovery of maybe 100ug of hGH from each pituitary. MAYBE. So for a 100iu (about 33mg) kit, you're looking at a lot of stiffs... not really cost effective.

From a recombinant E.coli strain:
-put the gene for hGH behind a promoter which is easily inducible. Quite often this is the lacZ promoter which is easily manipulated and is activated very strongly in the presence of lactose.
-transform E.coli with this plasmid, or integrate the gene construct (i.e. lacZ:hGH). It would be preferable to use a high copy-number plasmid. This sort of transformation could be also done with a baculovirus expression system over insect cells, or in yeast. E. coli would be the easiest to work with as far as total proteome, however, as many strains exist with a minimalized proteome of only perhaps 1,000-10,000 proteins instead of hundreds of thousands in mammalian cells.
-Grow the cells and induce the promoter (add lactose to the growth medium). This will stimulate mega-levels of expression of your target protein.
-Lyse the cells gently (i.e. by sonication or by addition of lysozyme). NOW, if your protein was tagged with an immune epitope (e.g. myc, HA, etc. can be added to the protein via genetic constructs, and they won't necessarily affect the function of the protein) you can run this over an immunoaffinity column - still less expensive than the equipment for large-scale prep HPLC, but maybe hundreds of thousands instead of millions for set-up costs - and have highly pure bound GH in a single step.
-wash away contaminating proteins with low-salt elution buffers.
-elute your hGH in high salt elution buffer, lyophilize the eluate.
-re-dilute, assay for protein purity and content, aliquot into vials, lyophilize, seal and distribute with vials of appropriate diluent.

Ferment 10,000 L of culture and you might get yourself kilograms of hGH in a single pass (with a large enough column). THAT is cost effectiveness.

-M

 

[gorilla_boy]

Gorilla_boy, I do this sort of shit for a living. I know what I'm talking about. Don't call me a defensive asshole when you're the one taking offence at a small, calm statement. Bullshit like this human pituitary extraction you're promoting is how rumours get started and live on as urban legends you tell your kids: "don't use GH - it's from dead bodies' pituitaries!"

<snip>
-M

-You called me an idiot in an otherwise rational discussion. You had it coming.
-I'm not promoting anything, I was looking for reasonable discussion from someone who actually knew what he was talking about.
-Thank you for a great explanation, you obviously do know what you're talking about.

 

[pharmguy]

The process of extracting GH from cadavers is going to be far more expensive then bacterial manufacture, no way its cadaverous origin
PG

 

[Sigmund Roid]

Ten years ago I have seen Russian amps of cadaver GH. It looked horrible, it was a brownish lyophilisate. Furtunately the owner threw them away after my warning.

I am quite sure that Jin is from E-coli. Anke has been tested and proved to be not from cadavers

 

[ironmaster]

It's good that you fellows agreed to have a rational discussion. This is an interesting post. We should be like family here.

Gorilla....you seem like a smart guy, and it sounds like you have a reason to distrust this brand. So what is it? As you and others pointed out, cadaver extracted GH was used in the past to treat several illness (people with non-functioning pituitary glands due to radiation, for example). And it was the subject of a number of research studies (see Medline for abstracts). And some of it did get into the sports and weight training world. But, man, that was a long time ago. The last diagnosed C-J case linked to cadaver GH was maybe 30 years ago. But you are right.....C-J disease did kill the market, legal and otherwise, for extracted GH.

I'm still pissed that Alzedo and his wife blamed his health problems on steroids.....really crusaded....when it the GH that really got him.


Anyway, I guess anything is possible. I've used 3 different brands of Chinese without complaint, but several of my friends have had allergic reactions to the same stuff.
I think the problem may actually be in the handling, not the manufacter. Chinese GH left to get warm and go bad is worthless at best, if not flat out dangerous. I have only used chinese GH that I received direct from the factory on ice.

Hey, Dr. M, what language is that you are speaking LOL. I'm happy to meet someone who understands this stuff. You know what's interesting.......seems almost every pharmaceutical company uses e-coli for the base except Serono. Serono uses mammalian proteins (rat) and "injects" human DNA into the molecule. Why are they different, and is that why Serostim doesn't need to be refrigerated prior to reconstitution?

 

[Quadsweep]

Who exactly makes Jinotropin?

GenSci makes it and it is rHGH which means its recumbiant which means its manufactured. They are a real deal company and their HGH is awsome.

Quad

 

[supertech69]

Wow, I must say Dr. M that was one of the most impressive posts I've seen on this board. You obviously know what you're talking about. It's nice to see an educated response once in a while instead of "just because that's how it is".