Well it's been several years since I researched it. Here are two articles about this subject, both by MD's that specialize in the field.
Both say evening injections are best.
Dosage
The most common dose is one unit of HGH taken daily in the evening, close to bedtime, although the time of day is probably not that important as long as daily doses are taken at approximately the same time of day. See detailed directions on filling the syringe below. The minimum cost effective dose for benefit is one unit daily for at least 4 consecutive days per week. Based on many years of experience, a 4-day schedule has been found to be the minimum dosing to achieve cost effective benefit.
Some patients take 1.5 or 2 units daily for added benefit at higher cost. More than 2 units daily are not recommended and may cause side effects. Syringes used to add diluent can hold a total of 3 mL and the syringes used for injection hold a total of 1 mL.
Alternate Dosing Option
Some patients choose to administer half the daily dose of HGH at bedtime and half on arising in the morning. There have been anecdotal reports of slightly increased benefit with the same total daily dose, although added benefit is doubtful and the number of injections is doubled.
If you decide to split your daily doses, taking half at night and half in the morning, you will need twice as many syringes. If you desire to use this twice-daily dosing option, please let us know and we will ship the additional number of syringes needed with no charge.
Evening versus morning injections of growth hormone (GH) in GH- deficient patients: effects on 24-hour patterns of circulating hormones and metabolites
JO Jorgensen, N Moller, T Lauritzen, KG Alberti, H Orskov and JS Christiansen
Second University Clinic of Internal Medicine, Aarhus Kommunehospital, Denmark.
Since serum GH in normal subjects displays a circadian variation with a major and consistent surge after the onset of sleep we examined whether the time of GH administration in GH-deficient patients had any impact on its action. Eight GH-deficient patients all underwent 3 4-week study schedules in random order: 1) evening (2000 h) sc GH injections, 2) morning (0800 h) sc GH injections, and 3) no GH administration. At the end of each period the patients were admitted to hospital for 24-h measurements of hormones and metabolites. For comparison, 10 age- and sex-matched healthy untreated subjects were hospitalized once under identical conditions. Mean (+/- SE) GH availability, i.e. the area under the curve (AUC; micrograms per L/12 h) for 12 h after injection was significantly greater after evening injection than after morning injections [83.3 +/- 25.4 (evening) vs. 46.0 +/- 10.6 (morning); P less than 0.01]. This might be due to higher skin and sc temperatures when in bed. The 2000-0800 h AUC after evening injection was similar to the corresponding AUC in the reference group. Mean 24-h serum insulin-like growth factor-I levels (micrograms per L) were similar after evening (189.8 +/- 2) and morning (179.5 +/- 5.3) injections (P = 0.8), but the latter displayed a circadian variation suggesting that a steady state had not been reached. Both were significantly lower than the stable reference value (248.4 +/- 3.6 micrograms/L). Blood glucose profiles after morning and evening GH did not differ from that of the reference group, whereas blood glucose decreased when the patients received no GH (P less than 0.01). Daytime (0800-2400 h) insulin levels were increased after morning injections (P less than 0.05). Nighttime levels of lipid intermediates were below normal in the untreated state and after morning injection (P less than 0.05), whereas nighttime blood alanine tended to be above normal after morning GH injection (P = 0.08). Highly significant inverse relationships between circadian lipid intermediates and both blood alanine and lactate concentrations were observed in the reference group and in the patients after evening injections. These relationships disappeared after morning injections. We conclude that the metabolic effects of sc GH injections are clearly influenced by the time of administration and that the closest similarity to normal hormone and metabolite patterns and relationships is reached by GH injection in the evening in GH-deficient patients.
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