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Whatman... .20 or .45 ?
- Thread starter anal itch
- Start date
fcfmuaythai
New member
I've heard that .45 is good for clarity, but .22 is best for sterilizing your gear.
anal itch
New member
I agree with this and have heard the same thing. BUT I would like to know what home brewers are actually using.
As a side note, every Fina kit I've seen comes with a .45 Whatman. Never a .20
I just got done making 100ml home brew EQ at 500mg/ml and started with a .20 Whatman. It took over an hour to filter 30ml then I finally gave up and switched to a .45. I finished the rest of the batch in 20 minutes. Isn't the BA supposed to be your sterilization mechanism?
As a side note, every Fina kit I've seen comes with a .45 Whatman. Never a .20
I just got done making 100ml home brew EQ at 500mg/ml and started with a .20 Whatman. It took over an hour to filter 30ml then I finally gave up and switched to a .45. I finished the rest of the batch in 20 minutes. Isn't the BA supposed to be your sterilization mechanism?
scoobysgym
New member
Yes I also made 120 mg eq and started with a 20 and after my arms were on fire i switched to a 45 I think the ba will sterlize anything Anyhow
Para_Shoot
New member
This is true. I went into the custom compounding pharmacy by my house and straight out asked the guy there if he could help me with the info on how to measure/mix three different powders together. He did help me and did all his calculations for me. He asked me how I was making my stuff, and I told him. The first thing he said was "what size filter you using?" I said a 45. He said I really need to use the 20, as thats what they use in the pharmacy. Do I use a 20? No, I use a 45. I made so much stuff already, which several people are testing out, and no one got any abcess, infection or anything else, and the juice comes out crystal.
anal itch
New member
moose11 said:
I've seen those. It looks like you can do large batches at once. I'm curious how well it works with the thicker oils though, and the housing is not reusable. I think they run $12-$15 for the filter.
The pump portion is the same exact part we use to bleed the brakes on my race bikes. It's called a Mighty Vac in the motorcycle scene. Kinda funny
rjl296
New member
.45 will eliminate all (99.99%) bacteria .
unless the can form endospores ie staph, which are about .3 micron if i rememeber correctly
odds of getting spores in there, slim-nil-
is it possible ?
sure it is
that being said, i always used a .2 when i made things
in addition, BA is not for really sterilizing, its to prevent bacteria from multiplying (ie- you inadvertently add some, or dont remove all of it)
but BA + time will kill most bacteria - (doenst help if you want to shoot the gear now though)
unless the can form endospores ie staph, which are about .3 micron if i rememeber correctly
odds of getting spores in there, slim-nil-
is it possible ?
sure it is
that being said, i always used a .2 when i made things
in addition, BA is not for really sterilizing, its to prevent bacteria from multiplying (ie- you inadvertently add some, or dont remove all of it)
but BA + time will kill most bacteria - (doenst help if you want to shoot the gear now though)
mrdeeznuts
New member
.20 will take about 10 minutes of squeezing to fill up a 10ml vial
.45 will take about 45 seconds
the filter is to take out debri
the ba and baking will kill the bacteria
make the gear in a sterile enviroment, fill your vials via the filter, then bake your gear
.45 will take about 45 seconds
the filter is to take out debri
the ba and baking will kill the bacteria
make the gear in a sterile enviroment, fill your vials via the filter, then bake your gear
justinjones1963
New member
excellent post and excellent points.
hayabusa976
New member
hey anal itch,u mentioned race bikes?????u talking street or dirt??
[email protected]
New member
Is it?hard to beliveanal itch said:
P
pumpedpittbullpower
Guest
well if u want sterile use .22 micron filter.
also the ba and bb have NOTHING to do with sterile solution. It suspends the chemical in the oil and thats all it does.
Also the syringe filter and too much work. heating oil will ruin the membrane and certain temps.
Just use a stericup filter .22 in a glass bottle .
also the ba and bb have NOTHING to do with sterile solution. It suspends the chemical in the oil and thats all it does.
Also the syringe filter and too much work. heating oil will ruin the membrane and certain temps.
Just use a stericup filter .22 in a glass bottle .
justinjones1963
New member
I guess I'll add my 2 cc's
I have decided to double filter. The initial batch is usually around 50ml so I use a .45 for that. I let the stuff sit a few weeks and then when I am ready to pump out the individual 10ml vials I use for a cucle I use a .2u
Better safe than sorry.
I have decided to double filter. The initial batch is usually around 50ml so I use a .45 for that. I let the stuff sit a few weeks and then when I am ready to pump out the individual 10ml vials I use for a cucle I use a .2u
Better safe than sorry.
anal itch
New member
hayabusa976 said:
damn, haven't been here in a looooong time. I race street bikes on road courses like VIR, Road Atlanta, Barber, Daytona (with the infield), etc. That's what's kept me away from here all summer. I should be back around now as we only have one race weekend left for the season.
interleukin 1
New member
.2 micron is the Chemistry / Biopharm standard for sterility. Yes, it is true .45micron is faster to filter you "science experiments" and chances are it get most of the "stuff"..But there are worse things found in your kitchen that are a lot worse then bacteria and these types of pathogens are usually way smaller. Mycobacteria and virusus make common bacteria look like Ron Jeramy's crank....so they will pass through the .45 pore size with ease.
Stick with the .2 micron....have a little patients!
If filter time is that bad for you, maybe invest in a filter pump from a lab supply company website. ....and I do not mean GPZ!
Stick with the .2 micron....have a little patients!
If filter time is that bad for you, maybe invest in a filter pump from a lab supply company website. ....and I do not mean GPZ!
jagerbombme
BANNED
i was using and changed to .2 for the reasons stated that there are particles smaller than .45 not many i've heard, a few viral and bacteria. however i heard ba will possibly kill the bacteria, but if the dead bacteria are small enough, they'll still pass through the .45 and now theres a whole separate debate on whether injecting dead bacteria in you makes your body respond to it in a similair manner as antibiotics?
A
ALIN
Guest
anal itch said:
.20 will take you some time to pass thru.
BigBastard
New member
How much can you pass through a .20 filter if you use unsteril grapeseed oil från the grossery shop before you need to change to a new filter?
.45 was always fine for me.
the562goodguy
New member
i just made a batch for personal use. i first filtered it through the .45 and got an infection on my shoulder. then reheated it and re filtered it through a .45 again. same thing happened. so i filtered through a .22 and hit it yesterday. so far no redness just sore cuz its prop. but we'll see how that comes along. it was really a drag filtering thru the .22 and after filtering like 40ml its started leaking all over the place. i was looking into those stericup filtering units. has anyone ever tried these or anything other than the syringe filters?
pip88
Banned
yea i have the vacuum filters just make sure the oil is warm or it will take all day.
anal itch
New member
I can't believe this thread is still alive after all this time! For an FYI, I've been using a .45 exclusively for the past several years and have not had any issues with sterility. Then again I'm super anal about the whole process so everything is very clean from start to finish.
I use a 0.22 -
AFAIK while the BA kills any bacteria/microbes, etc. - the 0.22 micron is needed to filter them out, with some microscopic particles able to pass through a 0.45.
The kits come with the 0.45 for the reason that the 0.22 will clog much much faster due to the binders and crap in the oil to be filtered.
It would be prudent - not always necessary however - to then run it through a 0.22.
As for time taken, i can get 100ml in 30-45mins using hands and a 0.22 filter.
If it were just myself using it then i may use a 0.45, if others do too i believe one needs to be stringent with sterility.
JMO
Basically .45 for oil is just fine and is perfectly sterile. .20 is for water based gear. I have been homebrewing for 4 yrs and never used anything but .45 for oils and .20 for waters. Also if you buy sterile filtered oil in bulk this cuts time by way more then half. Then all you have to filter is the powder you heat then run like 5cc of the sterile filtered oil back through the filter at the end to flush any gear left in the filter
I've been using the 20 to filter 2 carts. It's a killer on the fingers and forearms and I run it through 3 times after heating it in a hot water bath. I normally go through 2 filters. It takes about 3 hours to filter 40 ml. BUT comma I've never had a painful shot except for the usual nicking a vein or taking a rookie hesitant hit.
SlimJim300
New member
I've always used .45 and had no problems.
+1
wolfman87
New member
so this seems pretty much half and half to me. some people are saying .45 is fine and some say that .20 is the way to go. some even say that .20 is for water bases only. i don't want to bake mine so i'll take the extra time and use a .20 anyway. however it would seem that a .45 would be fine and the ba would clear it of any harmful stuff if it was left on the shelf for a week or so.
wnfrdsmith
New member
both are good as i used both and both have their own quality
sabarblatoe
New member
Great...I get to look like the perv here. To answer your question, the chick in scoobysgyms avitar is Chloe Jones.
If you filter a water based winny suspension you will filter all the active ingredient out. You can only filter water based solutions where the active component is suspended. For instance if you made home made bac water or acetic acid solution for use with IGF.
roidboyjnr
New member
ive only ever used .2 and i use a caulking gun and two screws to hold the syringe in place. if im filtering a bit i normally tape around the sides of the filter for support so it dont go pop on me. it still takes a bit of time but thats better than an infection and down time from the gym.
i also make sure its very warm for increased viscosity.
i also make sure its very warm for increased viscosity.
snutten_00
New member
I used the 0.45um Whatman and have had infection, the same product, I have filtered through 0.22um and no problems now ...
From now on, I am using 0.22um.
From now on, I am using 0.22um.
Yeah I am a fan of the 0.22um also. Sometimes I run it through the .45 first, then the 0.22 to make it easier to filter.
Double filtering is a waste of time.
I have used
. 2um and .45um and have never had problems with either with infections or what not. But if you want to be more safe go with the
. 2 but it's a bit getting over 50ml through a syringe filter
. 45um though I can do 100ml no problem
!ns
New member
used 0.45 whatman for first test cyp batch, spent about 10minutes for filtering 50ml of solution. i think BA and baking do its job for sterilize solution, never had any issue with this test C
read that baking in the stove can destroy hormone and lead to lost gear potency, next time i will not do this
read that baking in the stove can destroy hormone and lead to lost gear potency, next time i will not do this
Wow so many mistakes.
.45 doesnt sterilize gear
Baking doesnt sterilize gear
BA doesnt sterilize gear.
Why would u not use a .2 filter?
That is all u need. Baking also removes the BA and now u r growing bacteria in an already non-sterile environment, very dangerous. I am not being insulting just trying to help you and anyone else reading this thread.
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Cant get much clearer than that, period! Well said!
phalaenopsis
New member
According to Pharmacopea for sterylisation .22 um filter is needed. It's beacuse only .22 um filter will remove all bacteria form solution. .45 filters are used to remove non-soluable compuunds - only for claryfication. Also very critical point is transfering sterile filtered solution to vials and crimping. You must have sterile vials, stoppers and flip-tops. Solution must be transfered from receiver bottle in sterile environment (clean room). But until we don't have clean rooms in our houses we must be very, very cearful. BA doesn't sterylise solution. It prevent growth of germs. Sterylisaton methods are:
1.dry heat sterylisation (160*C for 2h, 170*C for 1h, 180*C for 30 min - time is measured from point when material achieve diserable temp.)
2. autoclaving (121*C 0,1 MPa overpressure for 15min)
3. filtering (.22 um filters)
4. radiation (gamma from Cs137 or Co60 radionuclids)
5. chemical (ethylene oxide gas, glutaraldehyde etc)
So we can take from this list 1,3 and 5 metod.
First method for glass vials and glass rciever bottles. Take large clean bowl, fill with distilled water add 2,3 drop of detergent (something you use for washing dishes) and wash in it your vials, bottles etc. After that flush your bowl with water and fill with distilled water. Flush vials and other glass until it won't shave. Take clean and washed with 70% isoprohyl alcohol metal tray, put on it upsidedown your glass. Put it to the oven and bake in desired temp. for appropirate time. Bottles must stay in normal position for few minutes to evaporate water, after that wrap thread with aluminium foil and put to the oven.
When glass is baking you can use 5 method to sterylise stoppers and flip tops by washing in glutaraldehyde/baking soda/water solution 2/0,3/97,7 % for 3h at standrad temperature or in 70% isoprophyl alcohol for same time. Afters 3h flush it in sterile water. Fill clean glass pressure bottle with distilled water to half volume and close it. Put it on water bath for 2h. When rubber and flip tops are flushed and wet I put it in opened bowl to cooling oven to dry it. Rubbers are stored after drying in closed bowl which is opened only when i take rubber to put it on vial and crimp.
Filling vials in air is risky but all steps which we done and BA in gear should make our gear safe. I heard about people which don't wash vials and rubbers, don't filter gear only bake it and thet are alive and haven't any infection. Allright. Junkies even use many times one neddle to IV injections and they are alive. But we are not junkies. We live healthy lifestyle and develop our bodies and we don't want HCV or any other nasty diseases.
Sorry for my language. I'm foreign and still learning
1.dry heat sterylisation (160*C for 2h, 170*C for 1h, 180*C for 30 min - time is measured from point when material achieve diserable temp.)
2. autoclaving (121*C 0,1 MPa overpressure for 15min)
3. filtering (.22 um filters)
4. radiation (gamma from Cs137 or Co60 radionuclids)
5. chemical (ethylene oxide gas, glutaraldehyde etc)
So we can take from this list 1,3 and 5 metod.
First method for glass vials and glass rciever bottles. Take large clean bowl, fill with distilled water add 2,3 drop of detergent (something you use for washing dishes) and wash in it your vials, bottles etc. After that flush your bowl with water and fill with distilled water. Flush vials and other glass until it won't shave. Take clean and washed with 70% isoprohyl alcohol metal tray, put on it upsidedown your glass. Put it to the oven and bake in desired temp. for appropirate time. Bottles must stay in normal position for few minutes to evaporate water, after that wrap thread with aluminium foil and put to the oven.
When glass is baking you can use 5 method to sterylise stoppers and flip tops by washing in glutaraldehyde/baking soda/water solution 2/0,3/97,7 % for 3h at standrad temperature or in 70% isoprophyl alcohol for same time. Afters 3h flush it in sterile water. Fill clean glass pressure bottle with distilled water to half volume and close it. Put it on water bath for 2h. When rubber and flip tops are flushed and wet I put it in opened bowl to cooling oven to dry it. Rubbers are stored after drying in closed bowl which is opened only when i take rubber to put it on vial and crimp.
Filling vials in air is risky but all steps which we done and BA in gear should make our gear safe. I heard about people which don't wash vials and rubbers, don't filter gear only bake it and thet are alive and haven't any infection. Allright. Junkies even use many times one neddle to IV injections and they are alive. But we are not junkies. We live healthy lifestyle and develop our bodies and we don't want HCV or any other nasty diseases.
Sorry for my language. I'm foreign and still learning
Wow that sounds like a commercial job to me.
phalaenopsis
New member
In my country where salary is low as in Africa whole gym collect cash and make gear for themsleve.
Well you should all collect cash and get an autoclave and save time and money with all those extra steps. Good luck to you bro!
phalaenopsis
New member
It isn't so inconvinient as you think. In 8h with filtering you can make even 200 10ml vials of few types of gear. It's enough for few months. With autoclave you still have to wash and dry vials and filter with .45 filter to remove non-soluable compuunds. Also pharamcopea recommends for oil solutions dry heat sterylisation which will melt stoppers or may degradate testosterone (don't know for sure, never tried baked gear). Autoclaving will bring oil to 121*C but it won't make saturated vapor beacuse oil is free of water. In autoclaving saturated vapor takes key role in sterylisation by hydrolising and denaturating enzyms. Thats mean you will only heat in autoclave your oil to 121*C for 15 minutes when for dry heat sterylization you need 160*C for at least 2h.
I'm not crazy about extra clean conditions and safety beacuse I konw people are injecting unsterile crap in to their vains and nothing happen but I wan't to have psychical comfort and I'm aware we are far away from legit labs.
Good luck to you bro!
I'm not crazy about extra clean conditions and safety beacuse I konw people are injecting unsterile crap in to their vains and nothing happen but I wan't to have psychical comfort and I'm aware we are far away from legit labs.
Good luck to you bro!
I am sorry. You are misquoting me or making incorrect statements on others. In 8 hours I have seen 10L mixed, filtered and bottled. I am going to bed but will help you in the am. You really should look into chemiclaving. And gear has to be filtered at .22 first.
Good luck to you also my friend.
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phalaenopsis
New member
I must misunderstand you. So when you wrote about autoclaving you had on your mind autoclaving only vials and rubberstops not final bottled product? Maybe you know degradating temperatures for different types of hormnes?
Regards
Regards
You will not degrade hormone by autoclaving after vials are capped. It's well below degradation temp.
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hospital?
or tatoo shop...
Or get your own autoclave.
or tatoo shop...
Or get your own autoclave.
snutten_00
New member
Wondering one thing only, Shoud i use sterile or nonsterile filters when I must filter finished product ?
AnthonyMarco
New member
When in doubt, sterile.
Non-sterile is awesome as long as you have a way to sterilize them since they are so much cheaper, but what happens is non-sterile have bacteria on/in the exit port which goes right in your vial.
In practice, the benzyl alcohol will kill any bacteria getting past filtration, but why risk it. There are some bacteria you might not survive.
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BA does not kill bacteria. It is there to keep it from growing.
AnonCanada
New member
Honestly, i moved from Whatman .20 and .45 filters over to a Nalgene Vacuum filter at .25 microns and am MUCH happier with the result. Cheaper over the long run an paying 3 dollars per whatman filter as well. And the Nalgene is much quicker.
That being said, if you only have access to the syringe filters, the .45 microns are fine.
That being said, if you only have access to the syringe filters, the .45 microns are fine.
No its not fine to use a .45. Do you have any idea the size of bacteria? Do you how much can pass through as small as .3?
This is horrible information please stop telling people this as it could cause and abscess.
AnthonyMarco
New member
Really? I have always been under the impression it killed it.
I have used .45 before with no problems whatsoever, but based on this it looks like I was just lucky.
I retract my previous post. Only use .2 or .22 micrometer pore size filters for filtering your injectables. It is really the ONLY way.
Don't risk your health/life to save a couple dollars or a couple days.
AnonCanada
New member
The compounding pharmacy that makes my HRT test uses a .45micron syringe filter. Used them for 10+ years with no abbesses. IMHO a .25 filter doesn't make up for the shortcomings of a truly sterile environment.
You can promote your .45 all you want, simply said its not the safest route. You cant argue that fact with your compounding pharm all you want. Do they autoclave after filtering?
Why cut corners on safety or yourself or others?
Just a poor decision.
AnthonyMarco
New member
I agree if you have some way to sterilize after filtering (autoclave/UV/etc.) .45 would be fine since the only reason to use .2 micron is to sterilize. But not many people have the means to sterilize after filtering, and not many people have a hepa laminar flow hood, so I will personally use .2 from now on just to be sure.
On a side note, I do want to experiment with pressure cooker sterilization though. I don't have any idea if it will work or the liquid will stay in the vial, but it would be nice if I can make it work.
My thought is if it can sterilize food, surely it can sterilize other things.
Yes it can and does work.
AnthonyMarco
New member
Awesome.
My son starts kindergarten Tuesday, and my wife starts teaching tomorrow, so I will have the house to myself all week for testing out approximate time frames for different packing densities. Surely I can plot some semblance of an exponential graph to be used as a guideline by others.
Thanks 4 T. I appreciate the replies, and am always interested in learning something new.
Anthony
Cool. Sounds like you have a better background in this stuff than I do. I hear if you have the money just buy an autoclave much less analysis required. Seal the door, flip the switch, 20 minutes later sterile vials.
ChemicalAmerica
New member
Hello my friends, I am new to this forum.
I filter all my oil stocks 0.45u before it.
We use two vacuum filtration units connected to a hand pump.
For the final product I use only 0.2u
My equipment is identical to the one below, two units of 250ml:
https://lh3.googleusercontent.com/E...v3PbnlXa_jW68iP1EhrKe979I-1F3kB_7Yke_2w=w1600
Sorry for the English translator.
I filter all my oil stocks 0.45u before it.
We use two vacuum filtration units connected to a hand pump.
For the final product I use only 0.2u
My equipment is identical to the one below, two units of 250ml:
https://lh3.googleusercontent.com/E...v3PbnlXa_jW68iP1EhrKe979I-1F3kB_7Yke_2w=w1600
Sorry for the English translator.
MaxxReps
New member
I pressure cooked agar slants in test tubes in a pressure cooker and the agar stayed in the tubes but they were only about 1/2 full. The trick is to let it cool fully before opening it (which I couldn't do as I PC'd them upright and layed them down to solidify after taking out of the PC and sealing them).
Problem with letting it cool is it sucks in unsterile air and if you have a pressure relief needle in the vial you could end up contaminating the vial.
You should pressure cook your sealed vials.
MaxxReps
New member
Have you done this? I've been thinking about this. The temp in a PC is lower than the boiling point of BB and BA and well under the smoke point of the oil, so the vials should not explode.
Would the heat affect the gear?
This would be the easiest way to make sterile preparations. I don't think you'd even need to prefilter the oil. Why doesn't everyone do this?
I had an autoclave to sterilize finished vials.
(Topic was Benzyl Alcohol Antimicrobial Efficacy, it's actually the reply to this post that is most relevant to this thread.)
ORIGINAL POST:
By: SV-1
Maybe this will help convince some people that they're not gonna die if they don't bake the sh*t out of their juice. Concentrate on clean conditions, proper technique, use a good filter (.2 micron) and you should be fine.
Development of a Multidose Formulation for a Humanized Monoclonal Antibody Using Experimental Design Techniques
"The efficacy of the preservative against various microorganisms was measured using a modified USP/EP PET (referred to as preservative screening test in this document). Tests were conducted at Microconsult Inc (Dallas, TX). In the procedure, formulations were tested against the following microorganisms: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. The 3 bacterial strains were inoculated together at a total concentration of ~105 cfu/mL, as were the 2 fungi. Samples were incubated for 7 days at room temperature (25°C), and the total bacterial and fungal counts were measured using a colony counter. The log reduction (LR) values for the bacterial and fungal counts were calculated as log (initial count/final count)."
"Results of the preservative screening tests showed that the formulations containing 0.75% and 0.5% benzyl alcohol are potential candidates to meet the USP/EP criteria (Table 4). Both formulations demonstrated a complete kill of the tested bacterial and fungal species after 7 days. For all other samples, either the total bacterial count after 7 days was too numerous to count, or no effective reduction in the bacterial count was observed."
"benzyl alcohol caused significant aggregation at high concentrations (≥1.0%); however, it was the most effective preservative in maintaining antimicrobial efficacy against bacteria and fungi."
REPLY TO THE ABOVE POST:
By: Snatch
this is good in conjunction with micropore filtration, but neither can replace the other. Micropore filtration isn't perfect, but it's damn good:
(Link I had to delete)
"Influence of Size, Shape, and Flexibility on Bacterial Passage through Micropore Membrane filters"
http://i.imgur.com/XcJQp.png
Note that these are the smallest bacteria they could come up with; filter your product through 0.45->0.22->0.1 and the CFU count will be so low your body has an extremely good chance of killing anything that gets in. No baking necessary; micropore filtration is routinely used to sterilize heat sensitive liquids.
Edit: While I have this open, here are some SEM images of bacteria caught in micropore filter for your enjoyment. Note that the first one actually makes it through just fine, so use BA.
The pic below on the left is the most informative part-
http://i.imgur.com/yMnRa.png
ORIGINAL POST:
By: SV-1
Maybe this will help convince some people that they're not gonna die if they don't bake the sh*t out of their juice. Concentrate on clean conditions, proper technique, use a good filter (.2 micron) and you should be fine.
Development of a Multidose Formulation for a Humanized Monoclonal Antibody Using Experimental Design Techniques
"The efficacy of the preservative against various microorganisms was measured using a modified USP/EP PET (referred to as preservative screening test in this document). Tests were conducted at Microconsult Inc (Dallas, TX). In the procedure, formulations were tested against the following microorganisms: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. The 3 bacterial strains were inoculated together at a total concentration of ~105 cfu/mL, as were the 2 fungi. Samples were incubated for 7 days at room temperature (25°C), and the total bacterial and fungal counts were measured using a colony counter. The log reduction (LR) values for the bacterial and fungal counts were calculated as log (initial count/final count)."
"Results of the preservative screening tests showed that the formulations containing 0.75% and 0.5% benzyl alcohol are potential candidates to meet the USP/EP criteria (Table 4). Both formulations demonstrated a complete kill of the tested bacterial and fungal species after 7 days. For all other samples, either the total bacterial count after 7 days was too numerous to count, or no effective reduction in the bacterial count was observed."
"benzyl alcohol caused significant aggregation at high concentrations (≥1.0%); however, it was the most effective preservative in maintaining antimicrobial efficacy against bacteria and fungi."
REPLY TO THE ABOVE POST:
By: Snatch
this is good in conjunction with micropore filtration, but neither can replace the other. Micropore filtration isn't perfect, but it's damn good:
(Link I had to delete)
"Influence of Size, Shape, and Flexibility on Bacterial Passage through Micropore Membrane filters"
http://i.imgur.com/XcJQp.png
Note that these are the smallest bacteria they could come up with; filter your product through 0.45->0.22->0.1 and the CFU count will be so low your body has an extremely good chance of killing anything that gets in. No baking necessary; micropore filtration is routinely used to sterilize heat sensitive liquids.
Edit: While I have this open, here are some SEM images of bacteria caught in micropore filter for your enjoyment. Note that the first one actually makes it through just fine, so use BA.
The pic below on the left is the most informative part-
http://i.imgur.com/yMnRa.png
