Please Scroll Down to See Forums Below 
fogg88
New member
For anyone who cares...
In a previous thread, I had mentioned how BigCat from bb.com claims that vitamin E will actually impair gains from PH/PS, assumedly by blocking the conversion process.
Well, I did some more digging and came up with a few abstracts that seem to indicate that vitamin E is actually necessary for this process, and that a deficiency of E is what causes problems. For your reading pleasure:
-------------------------------------------------------------------------------
Endocrinology 1986 Aug;119(2):464-9
Relative inactivation of steroidogenic enzyme activities of in vitro vitamin E-depleted human adrenal microsomes by lipid peroxidation.
Takayanagi R, Kato K, Ibayashi H.
The effects of lipid peroxidation and vitamin E on the steroidogenic activities of human adrenal microsomes were studied. The vitamin E content in the microsomes could be varied by treating the lyophilized microsomes with n-pentane, without affecting the steroidogenic enzyme activities. When the level of microsomal vitamin E in the adrenal was reduced to that in other tissues such as liver and kidney, NADPH-supported lipid peroxidation increased about 200-fold, and concomitantly, the steroidogenic enzyme activities decreased. After 5 min of the lipid peroxidation reaction, 17 alpha-hydroxylase and C17,20-lyase activities were inactivated to 13% and 18%, respectively, of the original activities. 3 beta-Hydroxysteroid dehydrogenase-isomerase and 21-hydroxylase, however, retained 89% and 84%, respectively, of the original activities. When vitamin E was reincorporated into the original activities. When vitamin E was reincorporated into the extracted microsomes, neither the lipid peroxidation reaction nor the inactivation of the enzyme activities was observed. These results indicate that the high concentration of vitamin E in adrenal protects the enzymes from oxidative damage, and that the microsomal C19 steroidogenic cytochrome P-450 activities are highly sensitive to lipid peroxidation. This suggests an association between adrenal lipid peroxidation and a decrease in C19 steroid synthesis with advancing age.
---------------------------------------------------------------------------------
Int J Vitam Nutr Res 1975;45(4):396-403
The effects of vitamin E deficiency on some enzymes of steroid hormone biosynthesis.
Barnes MM, Smith AJ.
The effect of vitamin E deficiency on three enzyme systems involved in steroidogenesis was investigated. No change was found in vitro activity of cholesterol side-chain cleavage enzyme in adrenal or testicular tissue, or of 11-beta-hydroxylase activity in adrenal tissue. Significantly less 3-beta-hydroxysteroid dehydrogenase activity was found in ovarian tissue from female rats given a diet deficient in vitamin E for 141 days, and in testicular tissue and adrenal glands from male rats given the deficient diet for 280 days. Testicular tissue from male rats given the deficient diet for 91 days showed considerably less 3-beta-hydroxysteroid dehydrogenase activity than did tissue from control rats, but the difference was not statistically significant.
--------------------------------------------------------------------------------
Thoughts, please??
In a previous thread, I had mentioned how BigCat from bb.com claims that vitamin E will actually impair gains from PH/PS, assumedly by blocking the conversion process.
Well, I did some more digging and came up with a few abstracts that seem to indicate that vitamin E is actually necessary for this process, and that a deficiency of E is what causes problems. For your reading pleasure:
-------------------------------------------------------------------------------
Endocrinology 1986 Aug;119(2):464-9
Relative inactivation of steroidogenic enzyme activities of in vitro vitamin E-depleted human adrenal microsomes by lipid peroxidation.
Takayanagi R, Kato K, Ibayashi H.
The effects of lipid peroxidation and vitamin E on the steroidogenic activities of human adrenal microsomes were studied. The vitamin E content in the microsomes could be varied by treating the lyophilized microsomes with n-pentane, without affecting the steroidogenic enzyme activities. When the level of microsomal vitamin E in the adrenal was reduced to that in other tissues such as liver and kidney, NADPH-supported lipid peroxidation increased about 200-fold, and concomitantly, the steroidogenic enzyme activities decreased. After 5 min of the lipid peroxidation reaction, 17 alpha-hydroxylase and C17,20-lyase activities were inactivated to 13% and 18%, respectively, of the original activities. 3 beta-Hydroxysteroid dehydrogenase-isomerase and 21-hydroxylase, however, retained 89% and 84%, respectively, of the original activities. When vitamin E was reincorporated into the original activities. When vitamin E was reincorporated into the extracted microsomes, neither the lipid peroxidation reaction nor the inactivation of the enzyme activities was observed. These results indicate that the high concentration of vitamin E in adrenal protects the enzymes from oxidative damage, and that the microsomal C19 steroidogenic cytochrome P-450 activities are highly sensitive to lipid peroxidation. This suggests an association between adrenal lipid peroxidation and a decrease in C19 steroid synthesis with advancing age.
---------------------------------------------------------------------------------
Int J Vitam Nutr Res 1975;45(4):396-403
The effects of vitamin E deficiency on some enzymes of steroid hormone biosynthesis.
Barnes MM, Smith AJ.
The effect of vitamin E deficiency on three enzyme systems involved in steroidogenesis was investigated. No change was found in vitro activity of cholesterol side-chain cleavage enzyme in adrenal or testicular tissue, or of 11-beta-hydroxylase activity in adrenal tissue. Significantly less 3-beta-hydroxysteroid dehydrogenase activity was found in ovarian tissue from female rats given a diet deficient in vitamin E for 141 days, and in testicular tissue and adrenal glands from male rats given the deficient diet for 280 days. Testicular tissue from male rats given the deficient diet for 91 days showed considerably less 3-beta-hydroxysteroid dehydrogenase activity than did tissue from control rats, but the difference was not statistically significant.
--------------------------------------------------------------------------------
Thoughts, please??
