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Jinotropin GH: Cadaver Salvage or Manufactured?
- Thread starter gorilla_boy
- Start date
DrM......slight clarification: (mouse, not rat...lol)
Serostim is produced by a mammalian cell line (mouse C127) that has been modified by the addition of the human growth hormone gene. Serostim, with the correct three-dimensional configuration, is secreted directly through the cell membrane into the cell-culture medium for collection and purification.
Serostim is produced by a mammalian cell line (mouse C127) that has been modified by the addition of the human growth hormone gene. Serostim, with the correct three-dimensional configuration, is secreted directly through the cell membrane into the cell-culture medium for collection and purification.
Dr. M
New member
ironmaster said:
This makes even more sense. Mouse cell lines are very, very easily modified to create human proteins - they actually have 'humanized' mouse strains that contain exclusively human metabolic enzymes (or other relevant pathways) so they can study drug metabolism, metabolic flux, etc. in mice as a prerequisite to human trials in some cases.
Now, since hGH is secreted, it likely has a secretory signal sequence somewhere in the N-terminus; being manufactured in a mammalian cell line means that this signal sequence targets it for secretion directly - no cell lysis needed!
Now, grow this stuff in a minimal medium like RPMI, and you're golden... virtually nothing to purify away; some might even go so far as to say that if you prepare a cell-free medium wash, it could be directly lyophilized and packaged!
Man, why didn't I realize that neuroendocrinology didn't apply to cell culture expression systems with a secretory signal sequence... Jeebus, I'm slipping here.
-M
Nice, doc.
I'm going to pick your brain some more. Back to that fina thread.....you know the one.......but a different question. I love/hate homebrew fina. Works great, but the cough, taste and impact on the kidneys worries me. I'm an old guy and used parabolan and finaject extensively in the past. Finaject is the esterified acetate version of trenbolone, like what we make, but there was no cough and no taste. I have to conclude that the difference is in the solvents. So which would you guess to be the culprit......benzl alcohol or benzl benzoate? It shouldn't be the BA, which is found in most of the steroids we use, or we would have the same problem with them. Right? Benzl benzoate, which can be used as a topical scabicide, sounds suspicious to me.
Wow, am I off topic or what!
I'm going to pick your brain some more. Back to that fina thread.....you know the one.......but a different question. I love/hate homebrew fina. Works great, but the cough, taste and impact on the kidneys worries me. I'm an old guy and used parabolan and finaject extensively in the past. Finaject is the esterified acetate version of trenbolone, like what we make, but there was no cough and no taste. I have to conclude that the difference is in the solvents. So which would you guess to be the culprit......benzl alcohol or benzl benzoate? It shouldn't be the BA, which is found in most of the steroids we use, or we would have the same problem with them. Right? Benzl benzoate, which can be used as a topical scabicide, sounds suspicious to me.
Wow, am I off topic or what!
Dr. M
New member
Actually, I haven't had any probs with up to 20-25% BB in any of my preparations (usually dilutions of high mg/mL gear like GTP 200mg/mL prop down to 50mg/mL; painless and quality).
I don't think it'd be the BB, but I'd really have to look more into what people are using in their kits. A high enough percentage of BA can be quite painful and/or destructive; in most gear that I've used the BA percentage doesn't exceed 0.9-2% as a bacteristatic/cosolvent.
If someone used 20-30% BA as a pellet solvent, I could see some problems with that...
Also, bear in mind that finaplix-H is manufactured to standards fit for cattle, not humans. Heavy metal contamination, detergent contamination, solvent contamination, etc. aren't issues in non-human, non-food/pharmaceutical grade products. I'm not saying any of this is ACTUALLY there, but it could be in some small amounts.
If I get a chance, I'll do some looking into it. Drop me a secure mail sometime at doctorm@elite and I'll remember to get back to you.
-M
I don't think it'd be the BB, but I'd really have to look more into what people are using in their kits. A high enough percentage of BA can be quite painful and/or destructive; in most gear that I've used the BA percentage doesn't exceed 0.9-2% as a bacteristatic/cosolvent.
If someone used 20-30% BA as a pellet solvent, I could see some problems with that...
Also, bear in mind that finaplix-H is manufactured to standards fit for cattle, not humans. Heavy metal contamination, detergent contamination, solvent contamination, etc. aren't issues in non-human, non-food/pharmaceutical grade products. I'm not saying any of this is ACTUALLY there, but it could be in some small amounts.
If I get a chance, I'll do some looking into it. Drop me a secure mail sometime at doctorm@elite and I'll remember to get back to you.
-M
