Why the AMES test does not say DNP is safe

[x_muscle]

With normal use DNP the core body temperature doesn't go up - the body is able to compensate by (excessive) sweating. So enzyme activation or tissue damage would only be an issue during an overdose of DNP.

that doesnt make since. changine the membrane proton gradient will release the heat to extracellular atmosphere.There is no upper limit to DNP's body temperature increase, meaning that one may literally "cook from the inside" if they take too much.

Now lets define too much..... It depend on each individual body, and the envioroment heshe lives in. so we can say 200mg or 100mg is a safe dose.


Osmotic thirst suppression during 2,4-dinitrophenol (DNP) hyperthermiain the dog.

Szczepanska-Sadowska E.

The effect of generalized body hyperthermia elicited by intravenous infusion of 2,4-dinitrophenol (DNP) on the reactivity of the thirst mechanism to osmotic stimuli was examined in conscious dogs. DNP increased deep body temperature by 1.53 +/- 0.18 degrees C in 18 out of 20 experiments. Impairement of thrist sensation was observed at the same time. The animals did not drink enough water to compensat for its total and evaporative loss. In cosequence water deficit developed, reaching maximum value of 2.7 plus 0.6% of body weight. The deficit was accompanied by an increase in plasma osmolarity, plasma protein concentration and hematocrit. A significant correlation between evaporative water loss and water deficit as well as between increase in deep body temperature and water deficit was found. The cellular dehydration developed in the course of DNP hyperthermia was higher by 3.3 +/- 0.6% of intracellular water (P less than 0.001) than that which was necessary to elicit drinking under conditions of normothermia. It is concluded that DNP hyperthermia changes the osmotic reactivity of the thirst mechanism so that the body fluids osmolarity is regulated at a higher level. This finding is discussed with regard to voluntary dehydration.


so you can die from dehydration without even fealing thirsty

 

[x_muscle]

There is evidence to suggest that elevation of body temperature may increase susceptibility of an animal to acute toxicosis. Exposure to high environmental temperatures or vigorous exercise is therefore contraindicated after disophenol administration.

 

[bigrand]

This goes without saying, keep hydrated and avoid extreme temps. If you work in construction in the summer, stay away. Keep the dose to 400mg for 7-10 and keep the fluids high and you should be fine, its not an extreme dose, i even have decent lifting workouts while on this (no cardio though). but thats just me.
Sleeping with a fan next to the head is a must also!

 

[juiceddreadlocks]

Those regions you speak of i believe are in the promotor region, responsible for begining translation of the gene, mutations in this area will change the AA sequence and the promotor will loose function=no active gene function.
Ill check, i havent gone into specific sections of the gene, only AAs around the C terminus to make a primer used turn on unactive mutant p53 (did some reading on the subject).

With DNP, the actuall result of DNP by itself is the change in permiability of the mito membrane, no electrical gradiant can be established because H+ leaks out. The problem DNP causes is secondary, free radical release from WAT, this is what could lead to a mutation, not DNP itself.

Yep, the theory is about 7 mutations to become malignant.
Some cases only require a few mutations if they are in the right places (ie a null mutation in p53 stops apoptosis).

I understand how DNP works. I was asking your understanding of science's understanding of the P53 gene.

 

[bigrand]

The part on DNP was for someone else.

The science behind p53 is becoming extensive. They can actually make a primer of wild type p53 (retro inverso using mirror image isomers to avoid degredation within the cell as if it were a foreign protein). They attatch this primer to a protein transfer domain (PTD) like the TAT protein (surface protein from HIV that faccilitates diffusion of a macro molecule through the cell membrane). Once inside the cell, the primer attatches to mutant p53 and can actually turn it back on! They have seen a SUBSTANTIAL increase in survival rates in highly terminal cancers like peritoneal carinomatosis (metastatic cancer to the peritoneum). Still in testing, but much more promise than current anti-neoplastic agents.

 

[OXANDRIN]

bigrand, is dnp stable in water...can a liquid version be made? sitting on some powder and capping is a painful mess for the little that i have

 

[bigrand]

Yup.
Easy way for me was to measure out 200mg, put it in a cup, mix with gatorade and shoot it. tastes like ass, but two shots a day for only 7-10 days aint bad.
I like it that way becuase i know im getting 200mg per.

 

[x_muscle]

bigrand, is dnp stable in water...can a liquid version be made? sitting on some powder and capping is a painful mess for the little that i have

you will dye your tongue yellow for couple of days

 

[bigrand]

yeah, and the taste isnt so breat, burns a bit on the way down but not bad! It is an acid though.

 

[bigrand]

Saw some DNP threads lately......this is a great read from the past.

BTW.....i figured out why cancer cells evade immune system detection. In many cases, the MHC I (major histocompatability complex I) that all cells in the body are SUPOSSED to produce (they are signals of intercellular damage and signal macrophages, like 69alpha's ass, to incude apoptosis and kill the damaged cell) are not present (likely a mutation to the genes that code for their expression extracellularly).
Not to be confused with MHC II on Macrophages, dendritic cells, and Tc cells.

With no "whiteflag", they reproduce untouched.