Why the AMES test does not say DNP is safe

[bigrand]

I guess ill finish out this current DNP cycle and then stick to thermorexin, there are way too many questions about DNP (looking through medline, i found hundreds of abstracts on DNP and its action on tumor cell, some seemed good, while others seemed bad, just too much shit for me to understand, so ill stick with the simpiler stuff).

 

[Ceebs]

Interesting reading.

If it is carcinogenic, I wonder how many cycles one would have to have done to accrue enough mutations to cause cancer. Dose, duration, frequency, etc, would obviously play a role...

 

[bigrand]

Very interesting reading here, we need a lot more of this on the board for us biology freaks.
Ive found nothing saying it IS carcinogenic, but then again, i can have some trouble deciphering the abstracts i read about DNP effects across the board.

 

[TraxZBT]

I guess its all a matter of time to see what the long term side effects are...

 

[MrMakaveli]

This def belonges in Best of Elite

 

[sk*]

This def belonges in Best of Elite

Noooo, not everyone can access that forum and the great responces won't keep coming ... not to mention the great information will be lost to most.

-sk

 

[MrMakaveli]

Noooo, not everyone can access that forum and the great responces won't keep coming ... not to mention the great information will be lost to most.

-sk

I met after its dead and buried...although you have a very good point

 

[Andy13]

Very interesting reading here, we need a lot more of this on the board for us biology freaks.
Ive found nothing saying it IS carcinogenic, but then again, i can have some trouble deciphering the abstracts i read about DNP effects across the board.

A simple in vitro expt could be done.. I think I could have such data gathered, but it would COST time.. ANd I'm not positive the demand is high enough...

A general tissue culture viability expt along with p53 Western would get us started... A Western will tell us if p53 is up-regulated or not. An increase in this protein is usually a good indicator of bad things occuring in the cell. Such things that would warrant DNA repair, apoptosis machinery.

If this data becomes interesting, co-treatment with anti-oxidants and/or other drugs may provide more feedback.

Like I said, I would be willing to see it done.. And it would not be any real expense to an appropriately equipped lab.. But time is money.

Andy

 

[sk*]

A simple in vitro expt could be done.. I think I could have such data gathered, but it would COST time.. ANd I'm not positive the demand is high enough...

A general tissue culture viability expt along with p53 Western would get us started... A Western will tell us if p53 is up-regulated or not. An increase in this protein is usually a good indicator of bad things occuring in the cell. Such things that would warrant DNA repair, apoptosis machinery.

If this data becomes interesting, co-treatment with anti-oxidants and/or other drugs may provide more feedback.

Like I said, I would be willing to see it done.. And it would not be any real expense to an appropriately equipped lab.. But time is money.

Andy

I think many people would be more than happy to cooperate ...

Maybe make a sticky about it and see how many people respond?

-sk

 

[bigrand]

Andy,
Would a change in p53 be the only other indicator of a cell line possibly becoming malignant? Im assuming that there are no other mutations caused by DNP, so it would have to be a change to a tumor suppressor gene...or some other important gene?
PS.... wouldnt that gene need to be downregulated or turned off if it is a tumor supressor gene?

All the abstracts i have been reading have been DNPs effects in more of a broad sense, such as its effects on various intra and extra cellular reactions, not on the DNA level, so i agree, this test of yours would be quite informative.