Start with no more than 1/4cc at one time. You can do that three times a day. You will want to use 1/2" 29g insulin syringes for pgf2a. These pins will work the best to inject though you will need to push hard enought to get into the muscle. If you injec subcutaneously you will get rid of fat but have not effect on the muslce. Very gradually increase the dosage so you can see how you are going to react to it. You can do it while on AS though most people get extreme soreness from pgf2a/AS so don't worry if your calves feel like ther are going to fall off after you inject.