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mrflexdiesel
New member
Flipping this a little bit. How would you go about making let's say Test powder into an injectable?
Raw Powders
- Thread starter JLAWz
- Start date
Go to conversionboard, and look at Budlight74's post on capping the var. The idea that you get a scale that measures .01 of a gram really doesn't help (I've got one myself) the reason is -if you put a catain amount on the scale it could read .01(10mgs) , but you could actually have .019(19 mgs)- before it would read .02, or .011 would still register .01, because it's accurat to .01 of a gram instead of .001 - make sense?? The is a large margin for error this way unfortunately.
Hardcoregrowth
New member
mrflexdiesel said:
There is a sticky at Bolex in the powder forum stating how to make many different things and amounts. It also has a powder calculator stuck also.
Dr. M
New member
mrflexdiesel said:
The short version of the story:
1 - weigh out your powder. For 10cc of 250mg/mL, weigh out 2.5g into a vial.
2 - Add about 0.9%-2% BA, 20% BB, and the rest oil (for 10mL, that means a drop or two of BA, 2 mL BB, and the rest - about 6.5-7mL due to the volume the gear takes up - USP oil). If you want to be exact with your concentrations, make up LARGER batches (say, 100cc at a time if you can afford it) and use a known-volume container. If you can buy a graduated cylinder or volumetric flask, that would be ideal.
3 - Allow to dissolve; heat slightly if necessary.
4 - Filter (0.22um sterile filter) into sealed sterile vial using extremely clean technique.
5 - Enjoy.
Now, this is a really short version... but this is just a side-note on the thread, right?
-M
mrflexdiesel
New member
Dr. M said:
Excellent! You are the man. By the way when you refer to USP oil is that like a sesame oil or propylene glycol. Sorry if I spelled that wrong. And is BA - benzy alchol and BB - benzyl benzoate? Thanks bro.
Dr. M
New member
USP oil is something like sesame oil, cottonseed oil, etc. which has been manufactured to a standard of the United States Pharmacopeia (USP). Usually this means it's handled very carefully from growth to pressing to bottling in order that it's not contaminated with anything particularly nasty (pesticides, immunogens, other pyrogens).
BA = benzyl alcohol, and BB = benzyl benzoate.
Adding more BB makes the gear more painless in my experience, especially with shorter ester test (eg. prop). BB is an excellent cosolvent which prevents crystallization upon injection; BA won't necessarily do this, and it can cause some tissue damage at the inject site if used at a high enough concentration. However, BB isn't bacteristatic, so it's good to throw BA in at 0.9%+; BA is also a great gear solvent so it's kinda dual-purpose.
-M
BA = benzyl alcohol, and BB = benzyl benzoate.
Adding more BB makes the gear more painless in my experience, especially with shorter ester test (eg. prop). BB is an excellent cosolvent which prevents crystallization upon injection; BA won't necessarily do this, and it can cause some tissue damage at the inject site if used at a high enough concentration. However, BB isn't bacteristatic, so it's good to throw BA in at 0.9%+; BA is also a great gear solvent so it's kinda dual-purpose.
-M
mrflexdiesel said:
Heres what I did...
Test Enanthate 10 gram conversion
Needed
10 grams test E
2ml Benzyl Alcohol = 5% BA
30.5 ml sesame oil
Syringes 3cc & 5/10cc
18 or 20 gauge needles
mixing Vial
Sterile Vial
Whatman sterile filter
1. Weigh out 10 grams of powder.
2. Place powder in vial.
3. Add BA to the vial.
4. Heat oil in the oven to help sterilize it, heat to at least 212 degrees F. I usually heat my oil at 275 to be sure, I put the oil in a muffin pan, just fill one of the indentions this is plenty for these experiments. Also heat more oil than you need, as you will not be able to get it all out of the pan.
5. Add oil to the vial, save 2 ml of oil in the syringe for later. Gently shake vial.
6. Heat mixture if necessary. I like heating my powder products, by placing the vial in a frying pan, and placing it on the eye of the stove.
7. Place a 18 or 20 gauge needle in the sterile vial attach Whatman sterile filter.
8. Place another needle in the sterile vial to relieve the pressure.
9. Draw out solution with a syringe, run though Whatman filter.
10. Take other syringe with 2ml oil, run through Whatman into solution.
Makes 40ml @ 250mg/ml
**you can lower the BA to 3.75% with 1.5ml and lower the oil slightly and add 5-10%BB. Painless indeed.
** I used grapeseed oil since it is the lightest I there is I believe.
Last edited:
Benzyl Alcohol is often the primary solvent and sometime the only solvent in a given formula. Its primary function is as a sterilizing agent. However, due to its solvency power it is often used to keep hormones in solution with oil or other vehicles. It is also one of the thinnest (that is best for viscosity reduction) The major drawback to this solvent is that it tends to make for painful injections if the concentration goes above 10%. (This is true for any low molecular weight alcohol). Less than this tends to be painless for most people.
Technical info: CAS # 100-51-6, Density is 1.05, Molecular weight is 108.14, Boiling point is 401f, Water solubility is 4.29g in 100ml.
Benzyl Benzoate is the next common solvent on the list. It is not nearly as strong and much heavier than the benzyl alcohol so benzyl benzoate is used primarily for its latent affects in the depot. Namely it helps keep the hormone in solution at the injection site after the more water-soluble benzyl alcohol has leached from the depot. benzyl benzoate is constructed of two benzene rings bridged by a carbonyl group, this making the solvent extremely hydrophobic. It is also painless at low levels and nearly painless at 15-20% for most people.
Technical info: CAS# 120-51-4, density is 1.11, Molecular Weight is 212.25 Boiling point is 614f, Water solubility is less then 1g in 100ml.
Technical info: CAS # 100-51-6, Density is 1.05, Molecular weight is 108.14, Boiling point is 401f, Water solubility is 4.29g in 100ml.
Benzyl Benzoate is the next common solvent on the list. It is not nearly as strong and much heavier than the benzyl alcohol so benzyl benzoate is used primarily for its latent affects in the depot. Namely it helps keep the hormone in solution at the injection site after the more water-soluble benzyl alcohol has leached from the depot. benzyl benzoate is constructed of two benzene rings bridged by a carbonyl group, this making the solvent extremely hydrophobic. It is also painless at low levels and nearly painless at 15-20% for most people.
Technical info: CAS# 120-51-4, density is 1.11, Molecular Weight is 212.25 Boiling point is 614f, Water solubility is less then 1g in 100ml.
Dr. M: Being that you are in the midst of earning your Ph.D. in biochem., I have a question for you:
How much testosterone in % (or any other AAS) would be lost in its filtration @0.22um?
How much smaller are AAS than the smallet virus? I aske these two questions since I am unable to find the molecualr size of testosterone, and since you should have a decent background in this field, I have turned to you
If you happen to come across the molecular size (in um) of testosterone (or another AAS) would you mind posting it? I would like to know how easily AAS pass through the 0.22um filter.
Thanks for any info!
How much testosterone in % (or any other AAS) would be lost in its filtration @0.22um?
How much smaller are AAS than the smallet virus? I aske these two questions since I am unable to find the molecualr size of testosterone, and since you should have a decent background in this field, I have turned to you
If you happen to come across the molecular size (in um) of testosterone (or another AAS) would you mind posting it? I would like to know how easily AAS pass through the 0.22um filter.
Thanks for any info!
Dr. M
New member
Diablo -
None of your testosterone should bind to the filter, but you may lose a bit due to retention of volume (i.e. that last little bit you can't get out of the filter). If this is a worry, make your test solution to about 9mL instead of 10mL, then after filtration take about a mL of sterile oil and run it through the filter to 'clean' it out. Testosterone is extremely hydrophobic, and nylon or PVDF filter membranes tend to bind highly charged protein species... testosterone is a totally different beast.
Viruses are measured in the tens (very rare, if any) to hundreds of nanometers (10E-8 to 10E-7 m). Viruses which thrive in mammalian cells are usually around 400-1200nm (0.4 to 1.2um). If you look at the molecular size of cholesterol (the nucleus of which is the basis for testosterone and other steroids), it's 44.5 Å (Ångstroms, which are 10E-10 m) in its longest dimension, if I remember correctly from my lipid biophysics classes. So, testosterone and other steroids, including their esters, are on average let's say almost a thousand times smaller than some of the smallest viruses which infect mammalian cells.
They whizz right through a 0.22um PVDF filter, while most (if not all) viruses and bacteria are retained by the filter membrane.
Does this clear things up? Karma if you're diggin the info...
-M
None of your testosterone should bind to the filter, but you may lose a bit due to retention of volume (i.e. that last little bit you can't get out of the filter). If this is a worry, make your test solution to about 9mL instead of 10mL, then after filtration take about a mL of sterile oil and run it through the filter to 'clean' it out. Testosterone is extremely hydrophobic, and nylon or PVDF filter membranes tend to bind highly charged protein species... testosterone is a totally different beast.
Viruses are measured in the tens (very rare, if any) to hundreds of nanometers (10E-8 to 10E-7 m). Viruses which thrive in mammalian cells are usually around 400-1200nm (0.4 to 1.2um). If you look at the molecular size of cholesterol (the nucleus of which is the basis for testosterone and other steroids), it's 44.5 Å (Ångstroms, which are 10E-10 m) in its longest dimension, if I remember correctly from my lipid biophysics classes. So, testosterone and other steroids, including their esters, are on average let's say almost a thousand times smaller than some of the smallest viruses which infect mammalian cells.
They whizz right through a 0.22um PVDF filter, while most (if not all) viruses and bacteria are retained by the filter membrane.
Does this clear things up? Karma if you're diggin the info...
-M
