Question on drug testing...

[cactus_jack]

Does anyone have any info on "fluorescence polarization immunoassay" testing? I have searched for awhile now and have come up empty. The most I know on this is that it is highly sensitive...however it does not state toward what. If anyone has any info, specifically dealing with AAS, please share it with me Thanks guys.

 

[Goldprospector]

It is just a type of drug test, But they still have to be specific on what type of drug they are testing for...If this is for getting a job somewhere, then I would not sweat it. It is very rare that anyplace does a AAS test unless it is some kind of sports association where AAS would get you thrown out of the cirriculum.



An immunoassay is a biochemical test that measures the level of a substance in a biological liquid, typically serum or urine, using the reaction of an antibody or antibodies to its antigen. The assay takes advantage of the specific binding of an antibody to its antigen. Monoclonal antibodies are often used as they only usually bind to one site of a particular molecule, and therefore provide a more specific and accurate test, which is less easily confused by the presence of other molecules. The antibodies picked must have a high affinity for the antigen (if there is antigen available, a very high proportion of it must bind to the antibody).
http://en.wikipedia.org/wiki/Immunoassay

Fluorescence Polarization Basics

Fluorescence Polarization detection technology enables homogeneous assays suitable for high throughput screening assays in the Drug Discovery field. The most common label in the assays is fluorescein. In FP-assay the fluorophore is excited with polarized light. Only fluorophores parallel to the light absorb and are excited. The excited state has a lifetime before the light emission occurs. During this time the labeled fluorophore molecule rotates and the polarization of the light emitted differs from the excitation plane. To evaluate the polarization two measurements are needed: the first using a polarized emission filter parallel to the excitation filter (Splane) and the second with a polarized emission filter perpendicular to the excitation filter (P-plane). The Fluorescence Polarization response is given as mP (milli-Polarization level) and is obtained from the equation.

 

[cactus_jack]

I have a police urinalysis coming up, though I dont know when. I BELIEVE this is the test they are using from what I have gathered. I am just wondering if I should stop AAS all together or just continue. I am not HUGE by any means so no suspicion would arise from my appearance. I was just nervous cause it seems this test is relatively new and I couldnt find much info. Thanks for the info Gold, much appreciated.

 

[amstel100]

I have a police urinalysis coming up, though I dont know when. I BELIEVE this is the test they are using from what I have gathered. I am just wondering if I should stop AAS all together or just continue. I am not HUGE by any means so no suspicion would arise from my appearance. I was just nervous cause it seems this test is relatively new and I couldnt find much info. Thanks for the info Gold, much appreciated.

If your being tested for the police academy they WILL test for juice!

 

[cactus_jack]

Well then, I better lay off for awhile haha. Thanks amstel