I am not here to start trouble with anyone, really, but... I have used bac water in one vial of IGF1-Lr3 that was used over the course of 2 months and I did not notice any degredation at all by the insane pumps I would still get while working out. Chemically, does it degrade over time with bac water? Probably. How long does it take to be useless when constituted with bac water? I don't know but it sure is longer than 48hours. Why didn't I use AA? Only cause I didn't have it. Would I have used AA if I had it? Yes, only because everyone says to.