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satchboogie
Guest
this website is dedicate do IGF-1 only..
come check it out and contribue...
www.getIGF.com
come check it out and contribue...
www.getIGF.com
Last edited:
Baywatch69
New member
Great idea, I'm looking to give IGF a run very soon, Added that site to my bookmarks, sure it will come in handy 
Trying to find out the potential sides before I start, dont really seem to be many, the cancer link scares me
Trying to find out the potential sides before I start, dont really seem to be many, the cancer link scares me
psychedout
New member
Firstly, IGF may extend the lifespan of humans, if you believe the results from rats can be generalized.
IGF-1 receptor regulates lifespan and resistance to oxidative stress in miceMartin Holzenberger1, Joëlle Dupont2, Bertrand Ducos1, Patricia Leneuve1, Alain Géloën3, Patrick C. Even4, Pascale Cervera5 and Yves Le Bouc1
Top of pageStudies in invertebrates have led to the identification of a number of genes that regulate lifespan, some of which encode components of the insulin or insulin-like signalling pathways1, 2, 3. Examples include the related tyrosine kinase receptors InR (Drosophila melanogaster) and DAF-2 (Caenorhabditis elegans) that are homologues of the mammalian insulin-like growth factor type 1 receptor (IGF-1R). To investigate whether IGF-1R also controls longevity in mammals, we inactivated the IGF-1R gene in mice (Igf1r). Here, using heterozygous knockout mice because null mutants are not viable, we report that Igf1r+/- mice live on average 26% longer than their wild-type littermates (P < 0.02). Female Igf1r+/- mice live 33% longer than wild-type females (P < 0.001), whereas the equivalent male mice show an increase in lifespan of 16%, which is not statistically significant. Long-lived Igf1r+/- mice do not develop dwarfism, their energy metabolism is normal, and their nutrient uptake, physical activity, fertility and reproduction are unaffected. The Igf1r+/- mice display greater resistance to oxidative stress, a known determinant of ageing. These results indicate that the IGF-1 receptor may be a central regulator of mammalian lifespan.
IGF-1 receptor regulates lifespan and resistance to oxidative stress in miceMartin Holzenberger1, Joëlle Dupont2, Bertrand Ducos1, Patricia Leneuve1, Alain Géloën3, Patrick C. Even4, Pascale Cervera5 and Yves Le Bouc1
Top of pageStudies in invertebrates have led to the identification of a number of genes that regulate lifespan, some of which encode components of the insulin or insulin-like signalling pathways1, 2, 3. Examples include the related tyrosine kinase receptors InR (Drosophila melanogaster) and DAF-2 (Caenorhabditis elegans) that are homologues of the mammalian insulin-like growth factor type 1 receptor (IGF-1R). To investigate whether IGF-1R also controls longevity in mammals, we inactivated the IGF-1R gene in mice (Igf1r). Here, using heterozygous knockout mice because null mutants are not viable, we report that Igf1r+/- mice live on average 26% longer than their wild-type littermates (P < 0.02). Female Igf1r+/- mice live 33% longer than wild-type females (P < 0.001), whereas the equivalent male mice show an increase in lifespan of 16%, which is not statistically significant. Long-lived Igf1r+/- mice do not develop dwarfism, their energy metabolism is normal, and their nutrient uptake, physical activity, fertility and reproduction are unaffected. The Igf1r+/- mice display greater resistance to oxidative stress, a known determinant of ageing. These results indicate that the IGF-1 receptor may be a central regulator of mammalian lifespan.
psychedout
New member
IGF-1 induces skeletal myocyte hypertrophy through calcineurin in association with GATA-2 and NF-ATc1
ANTONIO MUSARÒ*, KARL J. A. MCCULLAGH*, FRANCISCO J. NAYA†, ERIC N. OLSON† & NADIA ROSENTHAL*
* Cardiovascular Research Center, Massachusetts General HospitalEast, 149 13th Street, Charlestown, Massachusetts 02129, USA
† Department of Molecular Biology and Oncology, the University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, Texas 75225, USA
Localized synthesis of insulin-like growth factors (IGFs) has been broadly implicated in skeletal muscle growth, hypertrophy and regeneration. Virally delivered IGF-1 genes induce local skeletal muscle hypertrophy and attenuate age-related skeletal muscle atrophy, restoring and improving muscle mass and strength in mice. Here we show that the molecular pathways underlying the hypertrophic action of IGF-1 in skeletal muscle are similar to those responsible for cardiac hypertrophy. Transfected IGF-1 gene expression in postmitotic skeletal myocytes activates calcineurin-mediated calcium signalling by inducing calcineurin transcripts and nuclear localization of calcineurin protein. Expression of activated calcineurin mimics the effects of IGF-1, whereas expression of a dominant-negative calcineurin mutant or addition of cyclosporin, a calcineurin inhibitor, represses myocyte differentiation and hypertrophy. Either IGF-1 or activated calcineurin induces expression of the transcription factor GATA-2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of the transcription factor NF-ATc1. Thus, IGF-1 induces calcineurin-mediated signalling and activation of GATA-2, a marker of skeletal muscle hypertrophy, which cooperates with selected NF-ATc isoforms to activate gene expression programs.
ANTONIO MUSARÒ*, KARL J. A. MCCULLAGH*, FRANCISCO J. NAYA†, ERIC N. OLSON† & NADIA ROSENTHAL*
* Cardiovascular Research Center, Massachusetts General HospitalEast, 149 13th Street, Charlestown, Massachusetts 02129, USA
† Department of Molecular Biology and Oncology, the University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, Texas 75225, USA
Localized synthesis of insulin-like growth factors (IGFs) has been broadly implicated in skeletal muscle growth, hypertrophy and regeneration. Virally delivered IGF-1 genes induce local skeletal muscle hypertrophy and attenuate age-related skeletal muscle atrophy, restoring and improving muscle mass and strength in mice. Here we show that the molecular pathways underlying the hypertrophic action of IGF-1 in skeletal muscle are similar to those responsible for cardiac hypertrophy. Transfected IGF-1 gene expression in postmitotic skeletal myocytes activates calcineurin-mediated calcium signalling by inducing calcineurin transcripts and nuclear localization of calcineurin protein. Expression of activated calcineurin mimics the effects of IGF-1, whereas expression of a dominant-negative calcineurin mutant or addition of cyclosporin, a calcineurin inhibitor, represses myocyte differentiation and hypertrophy. Either IGF-1 or activated calcineurin induces expression of the transcription factor GATA-2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of the transcription factor NF-ATc1. Thus, IGF-1 induces calcineurin-mediated signalling and activation of GATA-2, a marker of skeletal muscle hypertrophy, which cooperates with selected NF-ATc isoforms to activate gene expression programs.
psychedout
New member
Here is some more. I am aware some of this literature may have to be stretched to apply to bodybuilders, but most of the literature regarding us is.
Muscle Protein Catabolism After Severe Burn: Effects of IGF-1/IGFBP-3 Treatment.
Annals of Surgery. 229(5):713, May 1999.
Herndon, David N. MD; Ramzy, Peter I. MD; DebRoy, Meelie A. MD; Zheng, Ming MD; Ferrando, Arny A. PhD; Chinkes, David L. PhD; Barret, Juan P. MD; Wolfe, Robert R. PhD; Wolf, Steven E. MD
Abstract:
Objective: To determine the effects of recombinant human insulin-like growth factor-1 (IGF-1) complexed with its principal binding protein, IGFBP-3, on skeletal muscle metabolism in severely burned children.
Summary Background Data: Severe burns are associated with a persistent hypermetabolic response characterized by hyperdynamic circulation and severe muscle catabolism and wasting. Previous studies showed that nutritional support and pharmacologic intervention with anabolic agents such as growth hormone and insulin abrogated muscle wasting and improved net protein synthesis in the severely burned. The use of these agents, however, has several adverse side effects. A new combination of IGF-1 and IGFBP-3 is now available for clinical study.
Methods: Twenty-nine severely burned children were prospectively studied before and after treatment with 0.5, 1, 2, or 4 mg/kg/day IGF-1/IGFBP-3 to determine net balance of protein across the leg, muscle protein fractional synthetic rates, and glucose metabolism. Another group was studied in a similar fashion without IGF-1/IGFBP-3 treatment as time controls.
Results: Seventeen of 29 children were catabolic before starting treatment. The infusion of 1.0 mg/kg/day IGF-1/IGFBP-3 increased serum IGF-1, which did not further increase with 2.0 and 4.0 mg/kg/day. IGF-1/IGFBP-3 treatment at 1 to 4 mg/kg/day improved net protein balance and increased muscle protein fractional synthetic rates. This effect was more pronounced in catabolic children. IGF-1/IGFBP-3 did not affect glucose uptake across the leg or change substrate utilization.
Conclusions: IGF-1/IGFBP-3 at doses of 1 to 4 mg/kg/day attenuates catabolism in catabolic burned children with negligible clinical side effects.
Muscle Protein Catabolism After Severe Burn: Effects of IGF-1/IGFBP-3 Treatment.
Annals of Surgery. 229(5):713, May 1999.
Herndon, David N. MD; Ramzy, Peter I. MD; DebRoy, Meelie A. MD; Zheng, Ming MD; Ferrando, Arny A. PhD; Chinkes, David L. PhD; Barret, Juan P. MD; Wolfe, Robert R. PhD; Wolf, Steven E. MD
Abstract:
Objective: To determine the effects of recombinant human insulin-like growth factor-1 (IGF-1) complexed with its principal binding protein, IGFBP-3, on skeletal muscle metabolism in severely burned children.
Summary Background Data: Severe burns are associated with a persistent hypermetabolic response characterized by hyperdynamic circulation and severe muscle catabolism and wasting. Previous studies showed that nutritional support and pharmacologic intervention with anabolic agents such as growth hormone and insulin abrogated muscle wasting and improved net protein synthesis in the severely burned. The use of these agents, however, has several adverse side effects. A new combination of IGF-1 and IGFBP-3 is now available for clinical study.
Methods: Twenty-nine severely burned children were prospectively studied before and after treatment with 0.5, 1, 2, or 4 mg/kg/day IGF-1/IGFBP-3 to determine net balance of protein across the leg, muscle protein fractional synthetic rates, and glucose metabolism. Another group was studied in a similar fashion without IGF-1/IGFBP-3 treatment as time controls.
Results: Seventeen of 29 children were catabolic before starting treatment. The infusion of 1.0 mg/kg/day IGF-1/IGFBP-3 increased serum IGF-1, which did not further increase with 2.0 and 4.0 mg/kg/day. IGF-1/IGFBP-3 treatment at 1 to 4 mg/kg/day improved net protein balance and increased muscle protein fractional synthetic rates. This effect was more pronounced in catabolic children. IGF-1/IGFBP-3 did not affect glucose uptake across the leg or change substrate utilization.
Conclusions: IGF-1/IGFBP-3 at doses of 1 to 4 mg/kg/day attenuates catabolism in catabolic burned children with negligible clinical side effects.
psychedout
New member
IGF-I stimulates muscle growth by suppressing protein breakdown and expression of atrophy-related ubiquitin ligases, atrogin-1 and MuRF1.
This is a pretty big one, a little to big to cut and post, but the direct link is:
http://ajpendo.physiology.org/cgi/content/full/287/4/E591
Expression, Regulation, and Function of IGF-1, IGF-1R, and IGF-1 Binding Proteins in Blood Vessels
Again, a little large, but here is the link.
http://atvb.ahajournals.org/cgi/content/full/24/3/435
Beneficial effects of GH/IGF-1 on skeletal muscle atrophy and function in experimental heart failure
Third one. Apparently it can reduce your chance of CHF. I think that stands for cardiovascular heart failure. My interpretation may be wrong though.
http://intl-ajpcell.physiology.org/cgi/content/full/286/1/C138
This is a pretty big one, a little to big to cut and post, but the direct link is:
http://ajpendo.physiology.org/cgi/content/full/287/4/E591
Expression, Regulation, and Function of IGF-1, IGF-1R, and IGF-1 Binding Proteins in Blood Vessels
Again, a little large, but here is the link.
http://atvb.ahajournals.org/cgi/content/full/24/3/435
Beneficial effects of GH/IGF-1 on skeletal muscle atrophy and function in experimental heart failure
Third one. Apparently it can reduce your chance of CHF. I think that stands for cardiovascular heart failure. My interpretation may be wrong though.
http://intl-ajpcell.physiology.org/cgi/content/full/286/1/C138
Güclü_oglan
New member
i've ran IGF-1 from pharmacies twice lately (both from sweden and from turkey) and i wouldn't advice you bros to do it unless very rich and won't bother a loss at a grand for a good cycle of IGF
got hungrier and i got more water in my muscles but nothing more
remember there are many theories of that IGF-1 can contribute to development of cancer
i'm skeptical but try it out and see if it works for you
got hungrier and i got more water in my muscles but nothing more
remember there are many theories of that IGF-1 can contribute to development of cancer
i'm skeptical but try it out and see if it works for you
wayneboard1
New member
bluehen said:
Why are you telling satch he is good looking?
psychedout
New member
IGF-1 induces human myotube hypertrophy by increasing cell recruitment.
Jacquemin V, Furling D, Bigot A, Butler-Browne GS, Mouly V.
CNRS UMR 7000 Cytosquelette et Developpement, Paris, France.
Insulin-like growth factor-1 (IGF-1) has been shown in rodents (i) in vivo to induce muscle fiber hypertrophy and to prevent muscle mass decline with age and (ii) in vitro to enhance the proliferative life span of myoblasts and to induce myotube hypertrophy. In this study, performed on human primary cultures, we have shown that IGF-1 has very little effect on the proliferative life span of human myoblasts but does delay replicative senescence. IGF-1 also induces hypertrophy of human myotubes in vitro, as characterized by an increase in the mean number of nuclei per myotube, an increase in the fusion index, and an increase in myosin heavy chain (MyHC) content. In addition, muscle hypertrophy can be triggered in the absence of proliferation by recruiting more mononucleated cells. We propose that IGF-1-induced hypertrophy can involve the recruitment of reserve cells in human skeletal muscle.
Jacquemin V, Furling D, Bigot A, Butler-Browne GS, Mouly V.
CNRS UMR 7000 Cytosquelette et Developpement, Paris, France.
Insulin-like growth factor-1 (IGF-1) has been shown in rodents (i) in vivo to induce muscle fiber hypertrophy and to prevent muscle mass decline with age and (ii) in vitro to enhance the proliferative life span of myoblasts and to induce myotube hypertrophy. In this study, performed on human primary cultures, we have shown that IGF-1 has very little effect on the proliferative life span of human myoblasts but does delay replicative senescence. IGF-1 also induces hypertrophy of human myotubes in vitro, as characterized by an increase in the mean number of nuclei per myotube, an increase in the fusion index, and an increase in myosin heavy chain (MyHC) content. In addition, muscle hypertrophy can be triggered in the absence of proliferation by recruiting more mononucleated cells. We propose that IGF-1-induced hypertrophy can involve the recruitment of reserve cells in human skeletal muscle.
wayneboard1
New member
bluehen said:Well did you see him in that other thread?, fucker
LOL...JK bro. This is a good idea as I have been doing a lot of my own reading on IGF and HGH. Never used either one and want to know everything about it before diving in. Good job satchhottie.
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satchboogie
Guest
bump
IGF first off doesn not cost 1k a cycle...maybe if you buy from a pharmacy...There is ONLY ONE manufacturer in the world with the patent on R3Igf-1 and that is Gropep out of Austrailia. I would never use media grade, as Receptor grade is the way to go. I have used IGF1R3 with fantastic results, at only 60mcg/day taken all directly into the muscle worked that day BEFORE my workout. Gained 12 lbs of LEAN mass in only 4 weeks...The only thing I stacked with it was 10-12iu humalog post workout which I think works very synergesticly with IGF.
If you can find a good source for Receptor grade you should be paying no more than 3---#&% per 1mg...I usually only use 2 mg per cycle...If you buy in bulk you will get a great deal, go in with a couple friends if your worried about loot.
If you can find a good source for Receptor grade you should be paying no more than 3---#&% per 1mg...I usually only use 2 mg per cycle...If you buy in bulk you will get a great deal, go in with a couple friends if your worried about loot.
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satchboogie
Guest
2sick said:
some questions about igf
does it increase strength or just pack on size?
do the gains stick around when you come off?
sides?
legal? im guessing no (just curious, not that i give a fuck)
will it show up in IOC drug tests?
from the way this stuff sounds id love to pick up a few kits, but i need to know the answers to these questions first
does it increase strength or just pack on size?
do the gains stick around when you come off?
sides?
legal? im guessing no (just curious, not that i give a fuck)
will it show up in IOC drug tests?
from the way this stuff sounds id love to pick up a few kits, but i need to know the answers to these questions first
Destroyer1986*
New member
That guy on the top right hand corner of the website looks a bit like Radar?
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satchboogie
Guest
|3ossman said:
come join www.getIGF.com discussion forum.
its a site dedicated to exactly the questions you need answered.
thanks.
Baywatch69
New member
Bump for www.getigf.com
Wow I didn't know that this IGF was from GroPep, I thought it was another pitch from OMEGA...Next time I better read the banner first Huh,
Well if it is from GroPep then it is the only good receptor grade IGF out. I will check the price and see if it is good or not, I get my IGF from a very reliable source who contracts from GroPep.
FOR DUMBASSES----DO NOT CONTACT GROPEP!!!!! I CANNOT STRESS THIS ENOUGH. do not contact them with any questions like a f----- idiot, this is a bad move and will ultimatley in the long run raise the price of IGF or discontinue it to there curtain distributors.....DO NOT CONTACT THEM with your questions that is what boards are for. They are a reputable company and will not tolerate lots of e-mails about questions instead they will start investigating...We have been through this on another board already
Well if it is from GroPep then it is the only good receptor grade IGF out. I will check the price and see if it is good or not, I get my IGF from a very reliable source who contracts from GroPep.
FOR DUMBASSES----DO NOT CONTACT GROPEP!!!!! I CANNOT STRESS THIS ENOUGH. do not contact them with any questions like a f----- idiot, this is a bad move and will ultimatley in the long run raise the price of IGF or discontinue it to there curtain distributors.....DO NOT CONTACT THEM with your questions that is what boards are for. They are a reputable company and will not tolerate lots of e-mails about questions instead they will start investigating...We have been through this on another board already
Baywatch69
New member
Putting this back on page one for those that might of missed it
wayneboard1
New member
satchboogie said:
Ok satch, Indy, whoever can help here, I do have a few questions about IGF. I have read the description of IGF on the website you posted satch. It describes it as mimicing insulin in the body. Pardon my ignorance here, but everything I have read about slin and my goals regarding the use of gear stear me away from slin. It's something I am obviously not educated enough to use, and the risks are too great FOR ME. This question may scream NEWBIE but is it as simple as IGF is insulin all be it a synthetic version?
It states it is a specific chain of amino acids, and that it can/should be used along with test, slin, etc, but then it says it mimics slin in the body so I am a little confused here. If someone in my situation wanted to stay away from Slin for the issues associated with it, is IGF basically the same thing with a different hat, or different alltogether and thus does not have the hazards associated with slin?
Thanks for helping an IGF newbie out.
wayneboard1 said:Ok satch, Indy, whoever can help here, I do have a few questions about IGF. I have read the description of IGF on the website you posted satch. It describes it as mimicing insulin in the body. Pardon my ignorance here, but everything I have read about slin and my goals regarding the use of gear stear me away from slin. It's something I am obviously not educated enough to use, and the risks are too great FOR ME. This question may scream NEWBIE but is it as simple as IGF is insulin all be it a synthetic version?
It states it is a specific chain of amino acids, and that it can/should be used along with test, slin, etc, but then it says it mimics slin in the body so I am a little confused here. If someone in my situation wanted to stay away from Slin for the issues associated with it, is IGF basically the same thing with a different hat, or different alltogether and thus does not have the hazards associated with slin?
Thanks for helping an IGF newbie out.
I can only put this simply as I am mobile right now and this wireless device does not have my documents on it....IGF-1 does mimic insulin the which is my it is called "IGF" Insulin LIKE Growth Factor, however it does not carry the risks of Insulin, as it is NOT in fact Insulin, it only acts like insulin in how it shuttles protein and enery into the cells, all I can remember off the top of my head right now. BUT, the reason I started IGF awhile back was because I was starting to experiment around with slin in that last cycle, knowing the risks....In my research I found that you could easily replace slin in your arsenal with IGF-1 to side steps those dangerous factors that come along with playing with slin. So I tossed the slin and GH aside, started IGF and the results are simply amazing......
With that said, the most powerful arsenal is of course AAS, SLIN, and IGF-1, all together, but for me, IGF was the replacement for slin, another risk I did not need in my life....I dont compete, why risk it.
Hope this helps....got your message too bro, yea caught up and doing better, thansk !!!
Indy-
wayneboard1
New member
indy69camaro said:
Right on bro, glad to hear you got some much needed rest and thanks for your mobile reply. I swear you are one of the most helpful guys on this board and your replies are a huge benefit to me so thanks man! OK OK enough of the
fest. lolI was glad to see that it only mimics slin and doesn't present the same dangers that slin does. I too will not ever compete so the risks of slin aren't at all worth it to me. Does the protein shuttleing effects IGF can have require any changes to your protein intake, or does the general 1 gram of protein per lb of body weight still apply?
I wonder how this would go with my test and var.
Wow, that could be a fun ride. I am really interested in how it can benefit tendons, ligaments, etc as I have always dealt with some sort of tendon issies in both my elbows. Nothing super serious but if it can help, that would be worth it to me in and of itself. Thanks again.
wayneboard1 said:Right on bro, glad to hear you got some much needed rest and thanks for your mobile reply. I swear you are one of the most helpful guys on this board and your replies are a huge benefit to me so thanks man! OK OK enough of the
I was glad to see that it only mimics slin and doesn't present the same dangers that slin does. I too will not ever compete so the risks of slin aren't at all worth it to me. Does the protein shuttleing effects IGF can have require any changes to your protein intake, or does the general 1 gram of protein per lb of body weight still apply?
I wonder how this would go with my test and var.
Thanks again.
I have not increased my protein intake over my normal routine of 200-300grams per day, so I would say it is more efficient as I am making larger gains with same amount of protein as prior.
I found some things wierd about that site getigf. I am having my doubts if it is infact sourced from Gropep.
For one Gropep says that their IGF cannot be left at room temperature and definitely not for up to 1 months. It is to be stored at just above freezeing. Gropep specifically states that their product cannot and should not be stored in your freezer.
Another thing is what www.getigf says should be a starting dose is way high, makes me think that they are refereing to Media grade and not recepto grade I mean starting media grade at 80-100mcg/day is overkill, ask anyone who has used IGF1 receptor grade I am experienced with this compound and I havent gone above 60mcg/day with fantstic results. This feeling is alos shared by all of the guys I know using the product although I know a few who have ventured into higher doses like 100-120 mcg/day and first off experienced headaches.
And last Gropep explains that you should not use BA to dilute your IGF, you should use a sodium chloride saline to reconstitue with.
All this leads me to belive that this is GETIGF is not sourced from GroPep. Not trying to be a dick but can anyone prove me wrong??? Satch?
For one Gropep says that their IGF cannot be left at room temperature and definitely not for up to 1 months. It is to be stored at just above freezeing. Gropep specifically states that their product cannot and should not be stored in your freezer.
Another thing is what www.getigf says should be a starting dose is way high, makes me think that they are refereing to Media grade and not recepto grade I mean starting media grade at 80-100mcg/day is overkill, ask anyone who has used IGF1 receptor grade I am experienced with this compound and I havent gone above 60mcg/day with fantstic results. This feeling is alos shared by all of the guys I know using the product although I know a few who have ventured into higher doses like 100-120 mcg/day and first off experienced headaches.
And last Gropep explains that you should not use BA to dilute your IGF, you should use a sodium chloride saline to reconstitue with.
All this leads me to belive that this is GETIGF is not sourced from GroPep. Not trying to be a dick but can anyone prove me wrong??? Satch?
jacked clown
New member
bump for 2sicks question
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satchboogie
Guest
and how would www.getIGF.com prove to you that they do in fact buy from GROPEP?
I dunno how they would prve it, but I am tempted to order 5 kits.....but the same thing was going through my head. I mean, I'm really not trying to bash the stuff.....but either they are giving wrong info about storage which could make the stucc useless or it isn't gropep. That is the same aftercare that igtropin has. and btw- I have run 2 igf cycles in the past.
Satch said it was Gropep in the begining of this thread.
I don't know how GETIGF woudl prove this BUT one of two things come to mine on my questions.
1 It may be Media grade bottled and labeled Receptor grade.
2 Or it is receptor grade By an underground company or nonlisceneced company and the doses they are pushing is to get you to buy more than you need in order for them to make more loot.
I am just going by facts, I get it for a bit cheaper from my guy but if he wasn't around and these guys ARE legit then it would be the ONLY other place I would know where to get This type of IGF and if my guy wasn't around I would invest if I needed it. BUT these questions plauge me and should immediatley be a concern to anyone who has used IGF receptor grade before... Especially the doseges claimed, and the storage. IGF cannot be stored at room temp, at least not GroPep's.
Just tring to clear this up, I am sure If I didnt' ask the questions now they would definitly come up later.
I don't know how GETIGF woudl prove this BUT one of two things come to mine on my questions.
1 It may be Media grade bottled and labeled Receptor grade.
2 Or it is receptor grade By an underground company or nonlisceneced company and the doses they are pushing is to get you to buy more than you need in order for them to make more loot.
I am just going by facts, I get it for a bit cheaper from my guy but if he wasn't around and these guys ARE legit then it would be the ONLY other place I would know where to get This type of IGF and if my guy wasn't around I would invest if I needed it. BUT these questions plauge me and should immediatley be a concern to anyone who has used IGF receptor grade before... Especially the doseges claimed, and the storage. IGF cannot be stored at room temp, at least not GroPep's.
Just tring to clear this up, I am sure If I didnt' ask the questions now they would definitly come up later.
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satchboogie
Guest
first off..
let me assure you www.getIGF's IGF-1 is of the highest quality!!!
our friends at OMEGALABS will attest that too as they too buy from the same place GROPEPE.
its media grade!
imossible to get receptor grade, so anybody claiming to sell you PURE receptor grade IGF-1 is misleading you! from my understanding, rececptor grade IGF-1 can only be used in lab settings as it cannot live out of fridge for very long... and besides, this is the highest quality media grade IGF-1 on the market today..
second...
GROPEP is a business...
and if you think for a second they're producing this product with sole intention to 'research' its use you are heavily mistaking!!!!
they sell the raw materials, and other products related to the manufacturing of IGF-1.
contact them directly if you wish.. they're not hard to find!
i do appreciate your concerns as spending our hard earned moneyshould be done with confidence.
thanks.
let me assure you www.getIGF's IGF-1 is of the highest quality!!!
our friends at OMEGALABS will attest that too as they too buy from the same place GROPEPE.
its media grade!
imossible to get receptor grade, so anybody claiming to sell you PURE receptor grade IGF-1 is misleading you! from my understanding, rececptor grade IGF-1 can only be used in lab settings as it cannot live out of fridge for very long... and besides, this is the highest quality media grade IGF-1 on the market today..
second...
GROPEP is a business...
and if you think for a second they're producing this product with sole intention to 'research' its use you are heavily mistaking!!!!
they sell the raw materials, and other products related to the manufacturing of IGF-1.
contact them directly if you wish.. they're not hard to find!
i do appreciate your concerns as spending our hard earned moneyshould be done with confidence.
thanks.
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They're the only ones with a patent................I'm sure any company with the same technology could make it, just illegaly. I belive it is made by rdna origin just like insulin and hgh. That has always pissed me off too, because all the shit costs the same to make as insulin..............I can walk into a pharmacy and get 1000iu's of humalog for $25 Canadian.
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satchboogie
Guest
bluehen said:
yes as far as we know, GROPEP is the only source for IGF raw materials.
word around the campfire is that a chinese UG lab is now starting to produce it too... but anything coming from china is questionable...
Well First I was under the impression that this was Receptor grade as it was stated in the beginning of this thread.
Correction their is Receptor grade IGF out there you just have to have the source who carries it. Your right it is extremely sensitive and connot live outside a cold cold fridge THIS is why I prompted these questions. If GETIGF is media grade then they should specifically state that on their site. It is misleading.
I do have access to Receptor grade and can provide the lab assays to prove it. Or I should say the company where I get it can provide these assays. Satch I am surprized you of all guys on this board cannot get a hold of true receptor grade.
Last I have contacted GroPep and know extensively about thier company this is why I had so many questions about the suppossed "receptor grade" getigf was selling. This is why I told people not to contact them anymore cuz they are a business and do not like people contacting them about their products if your not a lab it raises questions.
And just for the hell of it you know you can purchase Receptor grade from Gropep direct right??? You wouldn't belive the cost but it can be done, from what I hear I have never inqured about it to them myself but for 1mg it is 10x what you would pay from getigf.
Correction their is Receptor grade IGF out there you just have to have the source who carries it. Your right it is extremely sensitive and connot live outside a cold cold fridge THIS is why I prompted these questions. If GETIGF is media grade then they should specifically state that on their site. It is misleading.
I do have access to Receptor grade and can provide the lab assays to prove it. Or I should say the company where I get it can provide these assays. Satch I am surprized you of all guys on this board cannot get a hold of true receptor grade.
Last I have contacted GroPep and know extensively about thier company this is why I had so many questions about the suppossed "receptor grade" getigf was selling. This is why I told people not to contact them anymore cuz they are a business and do not like people contacting them about their products if your not a lab it raises questions.
And just for the hell of it you know you can purchase Receptor grade from Gropep direct right??? You wouldn't belive the cost but it can be done, from what I hear I have never inqured about it to them myself but for 1mg it is 10x what you would pay from getigf.
S
satchboogie
Guest
2sick good brotha..
yes you CAN indeed purchase receptor grade IGF directly form gropep.
but in all likelihood it will lose most if not all potency by the time it takes them to ship it from australia (where they're located).
i can also order a pound of steak online...
but when it gets to my house itll be no good..
unless of course i enjoy food poisoning.. lol
hope the analogy is understood..
thanks for youre feedback.
yes you CAN indeed purchase receptor grade IGF directly form gropep.
but in all likelihood it will lose most if not all potency by the time it takes them to ship it from australia (where they're located).
i can also order a pound of steak online...
but when it gets to my house itll be no good..
unless of course i enjoy food poisoning.. lol
hope the analogy is understood..
thanks for youre feedback.
So at the end of the day is the stuff from www.getigf.com any good? I am not sure where you guys are going with this. I have ordered two kits from them. Should I get a refund or treat it like media grade and do more injections throughout the day? Thanks, TT.
And 2sick I have sent them two emails asking getigf to make their comments and their arguments as to the validity of their claims. I also including links to this forum and bolex so that they know the BB community is aware of these discrepancies. I will keep you informed of any news.Thanks. TT
And 2sick I have sent them two emails asking getigf to make their comments and their arguments as to the validity of their claims. I also including links to this forum and bolex so that they know the BB community is aware of these discrepancies. I will keep you informed of any news.Thanks. TT
S
satchboogie
Guest
TT Guy said:
this should answer all you ask AND some:
http://www.getigf.com/IGF1DiscussionForums/tabid/53/view/topic/postid/264/forumid/1/Default.aspx#264
Last edited:
S
satchboogie
Guest
we've been getting alot of queries regarding this topic so www.getIGF.com contacted GROPEP and recieved some information which you will find worthy of reading.
straight from the manufacturer's website: GroPep scientists have produced Media Grade human LONG™R³IGF-I to provide an inexpensive yet high quality potent IGF-I analog for use as a growth factor supplement for serum-free or reduced-serum culture media.
Here is the info directly from the manufacturer's and patent holder's website:
Human LONG™R³IGF-I (Media Grade)
Purity: > 85 % (by N-terminal sequence analysis)
Molecular Weight: 9110 +/- 2 daltons – confirmed by Mass Spectrometry
N-terminal sequence analysis: 18 residues > 95 % single sequence
Biological Activity: Type 1 IGF receptor binding assay: ED50 > 15 ng/ml
IGF binding protein assay: ED50 > 200 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 10 ng/ml
Endotoxin: < 0.1 EU/µg
Human LONG™R³IGF-I (Receptor Grade)
Purity: >95 % (by HPLC and N-terminal sequence analysis)
Molecular Weight: 9111 daltons – confirmed by Mass Spectrometry
N-terminal sequence analysis: 18 residues > 95 % single sequence
Biological Activity: Type 1 IGF receptor binding assay: ED50 > 15 ng/ml
IGF binding protein assay: ED50 >200 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 10 ng/ml
Endotoxin: < 0.1 EU/µg
======================================================
So above you can see the purity is slightly different and the only other marked difference (if you can even consider it “marked”) is the molecular weight. The media grade may be off a dalton or two from the receptor grade.
there was a thread on www.getIGF.com by a respected Vet on several boards who stated “Only buy Lyophilized powder form of IGF-1!" This is the only way it comes and any other form is faked, or junk, or ruined”. Not true. Yes, Lyophilized powder is more stable than an improperly reconstituted form, but there are other factors that will play into that purchase. Perhaps the most import reason you don’t want to buy Lyo IGF is the fact that the IGF peptide is EXTREMELY delicate. It’s best to operate in a nitrogen environment, and when possible (and necessary) at slight vacuum (-25 kPa to be more specific).
Filtering requires some special skill and equipment also. (Taken from manufacturer’s site: Filter Sterilization of GroPep Peptides If sterilization is required, the peptide solution should be filter sterilized using a membrane with low protein binding; an example of this is the Millipore Millex-GV Cat. No. SLGV025LS.
Note that extreme care must be exercised in filtering dilute protein solutions because of the likelihood of adsorption to the filter. Growth factors require the same care in handling. Filtration for sterility is best done on either the 1 mg/ml stock solution or the 0.1 mg/ml working solution diluted in 10 mM HCl. It may be possible to filter more dilute solutions if a carrier protein is present but caution should be exercised.
Important Recommendations:
*Do not add the peptide to low protein or protein free media prior to filter sterilization.
*Use a filter membrane with low protein binding characteristics.
*Filter sterilize the IGF peptide separately at a concentration of 0.1 mg/ml (diluted in 10mM HCl) or greater.)
Chances that you’re going to fuck it up… ? Pretty damn good. This is why you want to buy your IGF-1 at www.getIGF.com. it comes properly reconstituted and in the proper medium with the right pH (done with HCL acid (10 mM HCl), not vinegar or many of the other “methods” at home methods.
we've also seen many posts from equally respected Vets touting the advantages of Receptor grade over Media grade and why the only IGF-1 with which to go is Receptor grade. They are equally mistaken and equally unqualified to render this opinion as the person referred to above. It’s not true. Plain and simple.
(1) From the manufacturer's website: GroPep scientists have produced Media Grade human LONG™R³IGF-I to provide an inexpensive yet high quality potent IGF-I analog for use as a growth factor supplement for serum-free or reduced-serum culture media. GroPep scientists have engineered this analog with the express purpose of increasing the biological activity of the IGF-I molecule. LONG™R³IGF-I is significantly more potent than human IGF-I in vitro. The enhanced potency is due to decreased binding of LONG™R³IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.
(2) Just as a point of clarification: we see posts about how IGF-1 not working for someone so they think they were scammed. Chances are, a large percentage of these posts are a result of damaged IGF and not necessarily a scam. Although a small amount of these people may have been scammed, IGF-1 is extremely delicate. Improper reconstitution, storage, shipping methods, excessive vibration, buying it 2nd, 3rd, 4th -hand, etc... can all cause the IGF peptide to erode. This is why it is critical that you know the source of the IGF and how it was reconstituted. At www.getIGF.com you dont have those concerns! Our IGF-1 is properly handled and more carefully reconstituted.
(3) we contacted Gropep and the explaination they gave goes like this: The purity of the MG is actually supposed to read >85% [so editted above]; however, most of the media grade actually far surpasses that standard and is not much different that RG, only that they cannot guarantee the purity standard exceeds that of the receptor grade , and thus, sell it as media grade. The letter from Gropep also was sure to state at the very end "Be aware that NO GroPep product is approved for use in humans."
=======================================================
in addition, ive spoken with a sales rep at GROPEP and this phone converstaion was summarized as such:
receptor grade IGF-1 is extremely sensitive to temperature conditions and mishandling. it can literally be destroyed in a matter of minutes in heat and/or when violently shaken or mishandled.
Receptor grade is just much more pure than media grade so lower doses work.. .20mcg-40mcg a day. but you pay for that! HEAVILY! Most texts we've examined state that for bodybuilding media grade is more than you would ever need.
The bottom line..
i doubt youll find any receptor grade IGF-1 in the u.s until GROPEP has it in their interest to spend millions of dollars on proper transportation of IGF-1 from australia to the u.s... not happening soon i can assure you of that.
at www.getIGF.com you are guaranteed the highest quality IGF-1 period! I cannot stress enough how much effort is put to insure the IGF-1 is properly handled, transported, reconstituted, and shipped to you.. the customer!
we appreciate the time you took to read this post and hope you found it informative.
sincerely,
admin.
straight from the manufacturer's website: GroPep scientists have produced Media Grade human LONG™R³IGF-I to provide an inexpensive yet high quality potent IGF-I analog for use as a growth factor supplement for serum-free or reduced-serum culture media.
Here is the info directly from the manufacturer's and patent holder's website:
Human LONG™R³IGF-I (Media Grade)
Purity: > 85 % (by N-terminal sequence analysis)
Molecular Weight: 9110 +/- 2 daltons – confirmed by Mass Spectrometry
N-terminal sequence analysis: 18 residues > 95 % single sequence
Biological Activity: Type 1 IGF receptor binding assay: ED50 > 15 ng/ml
IGF binding protein assay: ED50 > 200 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 10 ng/ml
Endotoxin: < 0.1 EU/µg
Human LONG™R³IGF-I (Receptor Grade)
Purity: >95 % (by HPLC and N-terminal sequence analysis)
Molecular Weight: 9111 daltons – confirmed by Mass Spectrometry
N-terminal sequence analysis: 18 residues > 95 % single sequence
Biological Activity: Type 1 IGF receptor binding assay: ED50 > 15 ng/ml
IGF binding protein assay: ED50 >200 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 10 ng/ml
Endotoxin: < 0.1 EU/µg
======================================================
So above you can see the purity is slightly different and the only other marked difference (if you can even consider it “marked”) is the molecular weight. The media grade may be off a dalton or two from the receptor grade.
there was a thread on www.getIGF.com by a respected Vet on several boards who stated “Only buy Lyophilized powder form of IGF-1!" This is the only way it comes and any other form is faked, or junk, or ruined”. Not true. Yes, Lyophilized powder is more stable than an improperly reconstituted form, but there are other factors that will play into that purchase. Perhaps the most import reason you don’t want to buy Lyo IGF is the fact that the IGF peptide is EXTREMELY delicate. It’s best to operate in a nitrogen environment, and when possible (and necessary) at slight vacuum (-25 kPa to be more specific).
Filtering requires some special skill and equipment also. (Taken from manufacturer’s site: Filter Sterilization of GroPep Peptides If sterilization is required, the peptide solution should be filter sterilized using a membrane with low protein binding; an example of this is the Millipore Millex-GV Cat. No. SLGV025LS.
Note that extreme care must be exercised in filtering dilute protein solutions because of the likelihood of adsorption to the filter. Growth factors require the same care in handling. Filtration for sterility is best done on either the 1 mg/ml stock solution or the 0.1 mg/ml working solution diluted in 10 mM HCl. It may be possible to filter more dilute solutions if a carrier protein is present but caution should be exercised.
Important Recommendations:
*Do not add the peptide to low protein or protein free media prior to filter sterilization.
*Use a filter membrane with low protein binding characteristics.
*Filter sterilize the IGF peptide separately at a concentration of 0.1 mg/ml (diluted in 10mM HCl) or greater.)
Chances that you’re going to fuck it up… ? Pretty damn good. This is why you want to buy your IGF-1 at www.getIGF.com. it comes properly reconstituted and in the proper medium with the right pH (done with HCL acid (10 mM HCl), not vinegar or many of the other “methods” at home methods.
we've also seen many posts from equally respected Vets touting the advantages of Receptor grade over Media grade and why the only IGF-1 with which to go is Receptor grade. They are equally mistaken and equally unqualified to render this opinion as the person referred to above. It’s not true. Plain and simple.
(1) From the manufacturer's website: GroPep scientists have produced Media Grade human LONG™R³IGF-I to provide an inexpensive yet high quality potent IGF-I analog for use as a growth factor supplement for serum-free or reduced-serum culture media. GroPep scientists have engineered this analog with the express purpose of increasing the biological activity of the IGF-I molecule. LONG™R³IGF-I is significantly more potent than human IGF-I in vitro. The enhanced potency is due to decreased binding of LONG™R³IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.
(2) Just as a point of clarification: we see posts about how IGF-1 not working for someone so they think they were scammed. Chances are, a large percentage of these posts are a result of damaged IGF and not necessarily a scam. Although a small amount of these people may have been scammed, IGF-1 is extremely delicate. Improper reconstitution, storage, shipping methods, excessive vibration, buying it 2nd, 3rd, 4th -hand, etc... can all cause the IGF peptide to erode. This is why it is critical that you know the source of the IGF and how it was reconstituted. At www.getIGF.com you dont have those concerns! Our IGF-1 is properly handled and more carefully reconstituted.
(3) we contacted Gropep and the explaination they gave goes like this: The purity of the MG is actually supposed to read >85% [so editted above]; however, most of the media grade actually far surpasses that standard and is not much different that RG, only that they cannot guarantee the purity standard exceeds that of the receptor grade , and thus, sell it as media grade. The letter from Gropep also was sure to state at the very end "Be aware that NO GroPep product is approved for use in humans."
=======================================================
in addition, ive spoken with a sales rep at GROPEP and this phone converstaion was summarized as such:
receptor grade IGF-1 is extremely sensitive to temperature conditions and mishandling. it can literally be destroyed in a matter of minutes in heat and/or when violently shaken or mishandled.
Receptor grade is just much more pure than media grade so lower doses work.. .20mcg-40mcg a day. but you pay for that! HEAVILY! Most texts we've examined state that for bodybuilding media grade is more than you would ever need.
The bottom line..
i doubt youll find any receptor grade IGF-1 in the u.s until GROPEP has it in their interest to spend millions of dollars on proper transportation of IGF-1 from australia to the u.s... not happening soon i can assure you of that.
at www.getIGF.com you are guaranteed the highest quality IGF-1 period! I cannot stress enough how much effort is put to insure the IGF-1 is properly handled, transported, reconstituted, and shipped to you.. the customer!
we appreciate the time you took to read this post and hope you found it informative.
sincerely,
admin.
indy69camaro said:
with exception of the price that is...take it from someone that knows igf pretty well, I think you guys are barking too loud, you want to pay for receptor grade, go for it, it's your money you're throwing away, you need media grade for stability and once again, you WONT see a diff between the 2 for what you want it for.
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