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testing sterility on your own

b_light

New member
Well this probably wont work or im way off but i was thinkin the other day. You know those little petrie dishes they use to test shit for bacteria and whatnot? Why not get some of those, shoot a little gear in them and let them incubate and see if your shits sterile or not? sounds feasible to me or is it more complicated then i am making it out to be?
 
There is a technique to preparing plates. Besides the plates you need hoops, a flame, an incubator.............

Also, identifying bacteria cultures is not always that easy, and some could settle on the plates in the short amount of time that it is exposed, from your skin etc...........


I once did a 'swab' experiment around the lab and grew all sorts of nasty looking plates, which were great for scaring the office ladies into washing their hands more.

Problem: most of them were normal bacteria that are on the skin.


Would you know which were which? The 'good' bacteria from the 'pathological ones'?

I had to do micro and do experiments I found it difficult and I got the microbiologists to help me with my little experiment.

It might be a fun experiment, but how reliable? It would depend on a lot of stuff.

I would think if the gear looks lumpy, cloudy, you have any concerns, don't use it.
 
Sure you can plate gear for sterility but keeping a sterile field while plating takes training and practice. An incubator is helpful but not absolutely necessary. I've grown bacterial plates on top of my refrigerator before. You just want dry, steady temperarures close to 100 degrees F.


To search for pathogens you need blood plates. If you find them, now you've concetrated pathogens on blood plates and you need special handling procedures to dispose of them without any exposure to yourself or others.

Since there is some danger, I would just rely on baking gear to ensure it's sterile.
 
Yeah you couldn't be 100% sure that you didn't introduce the bacteria from some other source.

And to that end some bacteria will might in the presence of "sterile" gear anyway.

There is bacteria that will grow on penicillin.

You can only do so much.
 
nydj66 said:
Sure you can plate gear for sterility but keeping a sterile field while plating takes training and practice. An incubator is helpful but not absolutely necessary. I've grown bacterial plates on top of my refrigerator before. You just want dry, steady temperarures close to 100 degrees F.


To search for pathogens you need blood plates. If you find them, now you've concetrated pathogens on blood plates and you need special handling procedures to dispose of them without any exposure to yourself or others.

Since there is some danger, I would just rely on baking gear to ensure it's sterile.


So true, or chocolate plates or some other growth accentuator as some of the nastiest bacteria are hard to grow.

You could sneeze on the plate and grow yourself a whole boat load of MRSA :)
 
b_light said:
Well this probably wont work or im way off but i was thinkin the other day. You know those little petrie dishes they use to test shit for bacteria and whatnot? Why not get some of those, shoot a little gear in them and let them incubate and see if your shits sterile or not? sounds feasible to me or is it more complicated then i am making it out to be?


I think you should just go out and bang 15 chicks without protection. If any come back pregnant next month you know your fertile.
 
Trendsetter21 said:
I think you should just go out and bang 15 chicks without protection. If any come back pregnant next month you know your fertile.


I dont know if hes talking about sterility of his gear or sperm production, but if it is sperm thats the idea i had
 
b_light said:
Well this probably wont work or im way off but i was thinkin the other day. You know those little petrie dishes they use to test shit for bacteria and whatnot? Why not get some of those, shoot a little gear in them and let them incubate and see if your shits sterile or not? sounds feasible to me or is it more complicated then i am making it out to be?

When I first saw the title of this thread I thought you were referring to testing to see if the man (not the gear) is sterile and I was going to post "well one easy way to find out...." heh heh
 
Tatyana said:
There is a technique to preparing plates. Besides the plates you need hoops, a flame, an incubator.............

Also, identifying bacteria cultures is not always that easy, and some could settle on the plates in the short amount of time that it is exposed, from your skin etc...........


I once did a 'swab' experiment around the lab and grew all sorts of nasty looking plates, which were great for scaring the office ladies into washing their hands more.

Problem: most of them were normal bacteria that are on the skin.


Would you know which were which? The 'good' bacteria from the 'pathological ones'?

I had to do micro and do experiments I found it difficult and I got the microbiologists to help me with my little experiment.

It might be a fun experiment, but how reliable? It would depend on a lot of stuff.

I would think if the gear looks lumpy, cloudy, you have any concerns, don't use it.


when i took micro we went to taco bell and burger king and swabed thier food then grew all kinds of shit. One taco bell we grew I think it was tricamoas(sp). or which ever one has a very distinct tortilla smell. it was funny cause the instructer just sat back and laughed when were all saying I will never eat thier again. he said that everywhere you go there are bacteria not matter how much you clean it even in the OR.
 
Trendsetter21 said:
I think you should just go out and bang 15 chicks without protection. If any come back pregnant next month you know your fertile.
Lol. I think your a little confused.
 
Tatyana said:
There is a technique to preparing plates. Besides the plates you need hoops, a flame, an incubator.............

Also, identifying bacteria cultures is not always that easy, and some could settle on the plates in the short amount of time that it is exposed, from your skin etc...........


I once did a 'swab' experiment around the lab and grew all sorts of nasty looking plates, which were great for scaring the office ladies into washing their hands more.

Problem: most of them were normal bacteria that are on the skin.


Would you know which were which? The 'good' bacteria from the 'pathological ones'?

I had to do micro and do experiments I found it difficult and I got the microbiologists to help me with my little experiment.

It might be a fun experiment, but how reliable? It would depend on a lot of stuff.

I would think if the gear looks lumpy, cloudy, you have any concerns, don't use it.


HEY GUYS, LOOKS LIKE WE'VE FOUND SOMEONE TO TEST OUR GEAR!! LOL j/k
 
Trendsetter21 said:
lol it was a joke
Nevermind, I guess I'm the confused one.

Some people around here do say things like that and actually are serious, believe it or not.
 
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