Re-Sterilizing gear question?

[bluehen]

What's the proper way of re-cooking gear to make sure it's sterile?

 

[Mr.X]

What's the proper way of re-cooking gear to make sure it's sterile?

how did it lose sterility in the first place?

adding BA and baking it is pretty much the standard way to go...

 

[bluehen]

how did it lose sterility in the first place?

adding BA and baking it is pretty much the standard way to go...

A friend has a 20 ml vial and wants to transfer it to two 10 ml bottles. He bought some pre-sealed sterilized vials but wanted re-cook it to make sure it's sterile. What temp and how much time should it be baked?

 

[hardrock]

If I remember correctly I used to bake Tren at 160 for 20 minutes. Been a while tho

 

[Indy]

What's the proper way of re-cooking gear to make sure it's sterile?

I only had to do it once, 20 minutes in oven, dont remember the temp. Bump for Flyingjer as he knows all this shit cold, or blue send him a pm, he'll take care of ya, he's good in the kitchen

 

[deezknuckles]

In graduate school, our standard procedure for sterilizing lab glassware was dry heat (baking) 1-2 h at 350 degrees F. THIS IS ONLY RECOMMENDED FOR OIL-BASED GEAR as water based gear would boil at this temp. Shouldnt be a problem with oil-based gear. Allow to cool to room temp in oven, cooling too rapidly can cause glass to shatter. Also, remove labels from vial as they will burn at this temp. Boiling point of Benzyl alcohol is~400 so go no higher than 350.

 

[hardrock]

In graduate school, our standard procedure for sterilizing lab glassware was dry heat (baking) 1-2 h at 350 degrees F. THIS IS ONLY RECOMMENDED FOR OIL-BASED GEAR as water based gear would boil at this temp. Shouldnt be a problem with oil-based gear. Allow to cool to room temp in oven, cooling too rapidly can cause glass to shatter. Also, remove labels from vial as they will burn at this temp. Boiling point of Benzyl alcohol is~400 so go no higher than 350.

350? This can't be. You're talking about sterelizing empty glassware. Not filled with juice right?

Doesen't anyone remember the Tren home brew craze from a few years back? I swear it was 160 or so. 350 just seems way high.

Anyone?

 

[bluehen]

350? This can't be. You're talking about sterelizing empty glassware. Not filled with juice right?

Doesen't anyone remember the Tren home brew craze from a few years back? I swear it was 160 or so. 350 just seems way high.

Anyone?

I know the glassware he bought was sterilized.

 

[bilter]

250 degrees for 20 - 30 min is typical. I like to preheat by letting it sit in a pot of water that I boiled first though (after taking the pot off the burner). This gets it up to 170 or so before sticking it into the oven.

 

[anal itch]

250 degrees for 20 - 30 min is typical.

This is what I've read for Tren. Check the melting points of the other gear as you don't want to exceed that temp. I think Test Prop's melting point is around 270. You can find this info in the chemistry forum.

Adding BA is your safest (but not the most pain free) option.

 

[Pikaberdot]

I always do 250ºF-260ºF for 45 minutes, and have never had any issues.

 

[Only1eagle]

yea stick to 200-230 to be safe. put a pin in (not in the liquid) to vent and bake for 20 minutes.

You should be ok.

 

[bluehen]

Thanks guys for the info.

 

[rjl296]

250 one hr