You have to mix all 5000ius at once... Get yourself a new vile, some bac water and some 1cc slin pins.
This is how I do it...I use two ways... 1. I put 1cc of bac water in the vile and 1cc of bac water in the amp with the powder and mix around. I then transfer the now mixed 5000ius into the vile with the 1cc of bac water using a normal 3cc needle. Now you have 2cc's of hcg equaling 5000ius. Now if you want 250ius all you have to do is use the FIRST 10 mark on your slinpin.
2. I use 500ius every 5 days... So I mix 1cc in the powder and then transfer it to the vile, I do not put that extra 1cc of water in the vile. And then the first 10 mark is 500ius for me.
I then wrap it in foil to protect from light and store in the fridge.. It should last 60 days plus.
Others will tell you to mix 10cc of bac water to the 5000ius of powder(9cc's in the vile, 1cc in the amp with powder).. If you do you will be injecting an extra 9/10 of water with each shot.... Its up to you.