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0.45 microns NOT GOOD ENOUGH!

S

Spidey

New member
Save yourselves from a possibly life threatening staph infection. Get a 0.22 micron syringe filter to convert your home brews. These filters will also sterilize "dirty" gear you may have acquired elsewhere.

A friend and I went in together on a box of synovex. We are both scientists and were very careful to keep a sterile environment. After filtering the finished product through a 0.45 micron filter in a sterile hood, we thought it was good. Well, my friend had the misfortune of using it first and developed a staph infection immediately.

Apparently, staphylococcus aureus is a small round bacterium that is small enough to go through 0.45 micron filters. To make it even worse, this bacteria forms spores that are thick walled and can withstand 200 to 300 degrees or straight alcohol so baking your product will not sterilize it. The only way to remove the bacteria and the spores is to filter with a 0.22 micron filter.

I can't find suitable syringe filters for sale one at a time so I am going to buy a box of fifty. I am also buying some sterile bottles.

If anyone out there would like to buy my extra syringe filters and/or sterile vials, PM me. I'm sure something can be arranged. I have no need for fifty.

I remind everyone that these items are completely legal to sell or own so I'm not breaking any laws or board rules by offering them.

-Spidey
 
thx9000 said:
www.anabolicfitness.com has them for $4.95ea.
Click to expand...
The filter from there is made of cellulose acetate. I have CA filters but can't get them to work with the oil, BB, BA mixtures. I don't know if the CA is dissolving or the pressure required to force the mixture through is too much but it is blowing right past the filter. I was going to buy a box of filters that have glass microfiber filters or PTFA.

Have you used the CA filters (0.2 micron)? can you put sesame oil, BB mixtures through them OK?

-Spidey
 
Dr.M said:
Sheeit, I can get 8 micron oil filters (WIC) :D
Click to expand...

I'd like to be there to watch you try and get home made tren through that .8.
If you are going to filter your tren you should use the .45 first and then the .2 because you will spend all day trying to get the tren through a .2 without prefiltering.
 
ulter said:


I'd like to be there to watch you try and get home made tren through that .8.
If you are going to filter your tren you should use the .45 first and then the .2 because you will spend all day trying to get the tren through a .2 without prefiltering.
Click to expand...

This is for car oil guys, I don't worry about bacteria in there. Most of the rest of the industry though is 20-45, I didn't notice the decimal place (not into tren just yet, if ever).
 
Flaming Heterosexual said:
Spidey, sorry to hear it.
I've been using .45 for as long as I can remember and never had a problem.
How much BA was in your solution? I usually use 3.5ml per 20ml of oil.

FH

My Homepage with links to cheap places to buy goods & Lab Results ;)
Click to expand...
This particular batch was only 14% BB and 1% BA. It doesn't matter though. BA will not kill spores. The spores are small enough to go through 0.45 micron filters. A 0.2 micron filter will catch them.

I tend to think maybe you (and many others) have just been lucky. Staph is a serious infection.

LOL, maybe I was just unlucky to have staph around when I was making the shit up.

-Spidey
 
i've gotten two infections previously from homebrew fina ...using a.45 micron filter. ...so IMO that .45 filter doesnt do jack shit. I will never touch fina again , by the way.
 
Flaming Heterosexual said:


Damn brother, again, sorry to hear it.
I hope he recovers well.

And you might be totally right... I am probably lucky.

Anyone know what Autoclaving the final solution (fina included) would do to the Tren?
It's 400 degrees of moist heat for 30 minutes.
I saw one on ebay for a couple hundred bucks.

FH

My Homepage with links to cheap places to buy goods & Lab Results ;)
Click to expand...
Probably wouldn't be good for it. 400 deg F is about 200 deg C and that's starting to get close to the boiling point of BA too. You would probably ruin the tren and evaporate a lot of your BA.

I tried heating my test prop solution to 100 deg C (212 deg F) for over an hour but that did not help. Not enough to kill spores. A knowledgeable friend told me that 500 deg or greater was needed to kill spores and I don't even know if he was talking deg F or deg C. BTW, 500 deg C = 932 deg F. I'm just going to get the damn 0.2 micron filter and filter the shit into a fresh sterile vial.

This sucks bigtime. My friend is on megadoses of amoxicillin and it's not a sure thing yet for him. He has abscess's in both ass-cheeks and one in his quad. I was supposed to start my test prop tonight but can't of course so this throws off my cycle a bit.

It really sucks because he tried to filter the stuff through a 0.2 micron filter initially but couldn't get it to flow through; to thick or something. We could have avoided all of this.:p

Maybe if I warm the oil a bit to make it thinner, it will go through the filter easier. I guess I'll try the cellulose acetate filter again. I hope CA isn't soluble in my oil mixture. The filters are only recommended for aqueous solutions.

-Spidey
 
I triple filtered through a 0.45 U filter and heated my stuff. I still had a lot of pain at the injection site the next day. I mean it was so bad that I couldn't use the shit!

Maybe I had the same problem and just need to filter through a 0.2 U filter???? I am affraid to take a shot of the stuff and I have a full bottle of it.

PM me with a price on the vial and filters....maybe we can work something out. I don't know if I should just throw my shit out....
 
OK. I found what I needed at getpinz.com. They have 0.2 micron PVDF filters (not CA) and they have 50 mL sterile vials. I ordered just what I needed so everyone can forget about that whole "I have extras if you want to buy them" thing:D

-Spidey
 
I would recommend filtering with a .45 filter, warming the solution and then filtering with a .2 filter.

The second filtering should catch pretty much everything. The key to getting it to pass through the membrane of the filter is to heat it. Get it as hot as possible to reduce the viscosity of the oil. However, don't be silly and burn it, just get it good and hot.

Also, go to www.getpinz.com for your filters/vials.

You'll thank me when the credit card bill arrives. :D
 
For the guys working at hospitals, research centres etc.:

You can also put your vials in an intense gamma ray from a radioactive source. Sounds stupid, but that is how syringes and needles are sterilised. This will kill all spores, viruses and bacteria without significantly harming the tren. Just look at the breads in the houses around Tjernobyl. They are still eatable, and are not rotten at all. Well, a bit dried out.

BTW, Spidey, what is the brand and type of your filters?
 
Actually, it's $8.00 for 2 filters, which means they are really $4 a piece. Also, they are a different type of filter (not the cellulose kind).
Just wanted to clear that up for everyone. I think this is a great thread and important point to make. Good job Spidey!
DAW

ulter said:
Holy smokes!!$8.00 for a filter??? That's it, I don't want to hear another word about how much AF charges for supplies. We sell that filter for $5.
Click to expand...
 
Sigmund Roid said:
For the guys working at hospitals, research centres etc.:

You can also put your vials in an intense gamma ray from a radioactive source. Sounds stupid, but that is how syringes and needles are sterilised. This will kill all spores, viruses and bacteria without significantly harming the tren. Just look at the breads in the houses around Tjernobyl. They are still eatable, and are not rotten at all. Well, a bit dried out.

BTW, Spidey, what is the brand and type of your filters?
Click to expand...
While I do work in a research center, I don't have access to a gamma source to sterilize my stuff. LOL, that would have just been too easy:p .

The filters I have ordered from www.getpinz.com are 0.2 micron PVDF filters. I looked up PVDF filters in the Fisher Scientific catalog and the description says "for aqueous or organic/aqueous mixtures". I hope they will be OK with the oil, BB, BA mixtures I want to filter. I am going to warm my oils a bit to make them less viscous before filtering. I hope that will do the trick.

BTW, Mvmax if you are reading, I ordered some 25 gauge 1.5" needles to inject my glutes. I'll be sure to let you know if it hurts more or less than the 1/2" 27 gauge I use currently.

-Spidey
 
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