Radioimmunoassay technique for detecting urinary excretion products after administration of synthetic anabolic steroids to the horse.
Xenobiotica 1978 Apr;8(4):197-206 (ISSN: 0049-8254)
Jondorf WR; Moss MS [Find other articles with these Authors]
1. Cross-bred and thoroughbred geldings were injected with veterinary doses of various synthetic anabolic steroids. Urines collected sequentially from treated animals were analysed, following solvent extraction, by radioimmunoassay using 19-[3H]nortestosterone and an antibody raised against a 19-nortestosterone immunogen. 2. Urinary excretion of 19-nortestosterone and/or its cross-reacting metabolites was detectable for various times after administration of different nortestosterone esters, as follows: phenylpropionate (400 mg), greater than 14 days; cyclohexylpropionate (100 mg), greather than 10 days; laurate (200 mg) greater than 50 days. After administration of the parent steroid (150 mg) cross-reacting compounds were detectable in urine for ca. 3 days. 3. Urinary excretion of esters of other anabolic steroids cross-reacting with the 19-nortestosterone antibody (e.g. 1-dehydrotestosterone and trienbolone) could also be followed by analysing solvent extracts of urines by the radioimmunoassay. Cross-reacting compounds in urine after administration of 1-dehydrotestosterone undecylenate (250 mg) and trienbolone acetate (75 mg) could be detected for greater than 35 days and greather than 5 days, respectively.
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I know, it's not much of help, because the study was done in 78, and on horses. But, on the other hand, the method of administration was injections, not implantation, also we can compare the resulst, to come up with approx numbers.
Please, don't take it, as I'm applying those for your practical use, what all this coming up with have to be definatelly taken with a grain of salt.
Anyway, 400mg of Durabolin - greater then 14 days.
------------ 75mg of TA ----------- greater then 5 days.
Detection times of Durabolin in humans is approx 12 month, so accordingly, TA would be 4-5 month.
Again, late discoveries of methods of detecting steroid metabolites that are stored in fat and leak over long period of time might greatly increase your chances to get busted.
Also, methods used on horses in 78 are kind of old...
If you will get busted, pay off your local butcher to provide receipts, that you kept eating tons of boar and at least a lb of cow's ears a day. J/K.
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