It depends on the test, the source, the ester, and the person. Some people are crippled by any shot of test prop, some people arent. Some people can handle test E at 500mg/ml, some cant. Test prop from one source might hurt like hell (like the first batch i ever made... 5%BA, 10%BB... hurt like hell... i still have like 10 bottles of it sitting around because i cant use it... after that, using EO and much lower BA, its painless.) There are alot of factors to consider. But in general, no, every shot shouldnt be painfull... even with prop, the more you shoot, the less pain you get from the shot because your body gets used to it usually.