Makes no difference how you mix it up in water...know how many IU's are in total powder in vial. If you resuspend , say, 18IU in 1ml of water, then to get 1 IU per injection, divide 1ml by 18, or on an insulin syringe, that would be .055 ml per shot. This is a wasteful way to resuspend, as you will always lose some of the fluid in the needle...so resuspend in 2ml, and give your self 11 of those little units on an insulin syringe (the .5ml variety). That will be 1IU per shot. AND, do this before you go to bed, not in the morning...HGH is used while sleeping, not while awake. And it has a half life of about 2 hours in the body! I started using this stuff at 60 years old, did 2IU per day, evening shots. Within 6 months, my skin had lost many of the age wrinkles, my memory was better, and my energy was higher. I felt and looked better. BE AWARE that this stuff goes bad very quickly in the refrigerator...I do not believe that, if you take 1 IU per day, the stuff is good at day, say 15...the half life is ticking away as soon as it is mixed. Better to share with a buddy to reduce the time from mixing to vial being empty.