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gurus only!!!! how AS( ESTERS) ARED MADE?or made from?
- Thread starter RICOSUAVE
- Start date
This is the general procedure, but it would be upscaled by pharmaceutical companies to produce mass quantities (eg reaction vessels instead of flasks). There is nothing special here about DHT. You could use testosterone as well.
(The test itself is made either from stigmasterol (from soybean oil) or cholesterol (from fat). The unwanted side chains from these compounds are cleaved in vats by microbes, and then the product is reduced to form test.)
Synthesis of DHT heptanoate was done by acylation of the 17ß-hydroxyl group of DHT. DHT (25 g) was dissolved in dichloromethane (250 mL). Heptanoyl chloride (16 mL) was added, together with 4-(N,N-dimethylamino)pyridine (3.75 g) as a catalyst. The flask was stoppered with an anhydrous calcium chloride tube, the entire apparatus was protected from the light, and the reaction mixture was stirred magnetically at regular intervals. After 1 week the reaction mixture was washed with sodium hydroxide (2 x 75 mL, 1 mol/L), hydrochloric acid (2 x 75 mL, 1 mol/L), and then deionized water until an aqueous layer of neutral pH was obtained. Evaporation of the organic layer resulted in a yellow, waxy solid that was further dried in a desiccator for several days. The product was recrystallized with the use of acetone/water and characterized by electron impact full-scan MS with an ion-trap detector (ITD 800; Finnigan MAT) coupled to a gas chromatograph (Model 5890A; Hewlett-Packard) fitted with a HP-1 methyl silicone capillary column (3). Purity of the compound was also assessed by nuclear (1H) magnetic resonance spectroscopy (AMX 400 NMR spectrometer; Bruker Spectrospin). The DHT heptanoate was prepared for injection (250 mg in 1 mL of arachis oil and benzoyl alcohol solution) at St. Thomas' Hospital, London
http://www.clinchem.org/cgi/content/full/43/11/2091
www.cuttingedgemuscle.com
(The test itself is made either from stigmasterol (from soybean oil) or cholesterol (from fat). The unwanted side chains from these compounds are cleaved in vats by microbes, and then the product is reduced to form test.)
Synthesis of DHT heptanoate was done by acylation of the 17ß-hydroxyl group of DHT. DHT (25 g) was dissolved in dichloromethane (250 mL). Heptanoyl chloride (16 mL) was added, together with 4-(N,N-dimethylamino)pyridine (3.75 g) as a catalyst. The flask was stoppered with an anhydrous calcium chloride tube, the entire apparatus was protected from the light, and the reaction mixture was stirred magnetically at regular intervals. After 1 week the reaction mixture was washed with sodium hydroxide (2 x 75 mL, 1 mol/L), hydrochloric acid (2 x 75 mL, 1 mol/L), and then deionized water until an aqueous layer of neutral pH was obtained. Evaporation of the organic layer resulted in a yellow, waxy solid that was further dried in a desiccator for several days. The product was recrystallized with the use of acetone/water and characterized by electron impact full-scan MS with an ion-trap detector (ITD 800; Finnigan MAT) coupled to a gas chromatograph (Model 5890A; Hewlett-Packard) fitted with a HP-1 methyl silicone capillary column (3). Purity of the compound was also assessed by nuclear (1H) magnetic resonance spectroscopy (AMX 400 NMR spectrometer; Bruker Spectrospin). The DHT heptanoate was prepared for injection (250 mg in 1 mL of arachis oil and benzoyl alcohol solution) at St. Thomas' Hospital, London
http://www.clinchem.org/cgi/content/full/43/11/2091
www.cuttingedgemuscle.com
Sigmund Roid
New member
Where the hell is the HCl buffer? They should have included an at least equimolar amount of say pyridine, dimethylformamide or something. No wonder the reaction takes 1 week.
DHT + hept. chloride -> DHT - heptanoate + HCl
HCl + DMF -> DMF(dot)HCl
So far my elementary school chemistry.
DHT + hept. chloride -> DHT - heptanoate + HCl
HCl + DMF -> DMF(dot)HCl
So far my elementary school chemistry.
PsychoPikey
New member
i dont think you can get an hcl buffer
well at A-level you couldnt
well at A-level you couldnt
