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aa needed to mix with igf-1 lr3
- Thread starter jvilma51
- Start date
CHEEZE1313
New member
heres how to make it...
1. Wash you hands thoroughly 2. Optional: wear alcohol treated exam gloves (rub your gloved hands together with 70% isopropyl alcohol on them until dry) 3. Using a sterile alcohol prep pad, swab the top of your sterile glass vial (into which the acetic acid solution will be held in) 4. Using the 10mL syringe with a ~20ga. needle on the end, draw up 7.5mL distilled water 5. Using the same syringe, now draw up 1mL vinegar 6. Remove needle from the syringe and discard 7. Attach 0.2-0.22um Whatman sterile syringe filter (do not touch the free end that will have a needle on it)
For Research Use Only 4
8. Put a new, sterile needle (~20 gauge) onto the free end of the Whatman filter (do not touch needle) a. Do not use the same needle on the Whatman that was used to originally draw up the unsterile vinegar and distilled water. 9. Put a ~20 gauge sterile needle into the top of your sterile glass vial to act as a vent 10. Inject the acetic acid solution into the vial 11. You are now done and should have sterile 0.6% acetic acid
1. Wash you hands thoroughly 2. Optional: wear alcohol treated exam gloves (rub your gloved hands together with 70% isopropyl alcohol on them until dry) 3. Using a sterile alcohol prep pad, swab the top of your sterile glass vial (into which the acetic acid solution will be held in) 4. Using the 10mL syringe with a ~20ga. needle on the end, draw up 7.5mL distilled water 5. Using the same syringe, now draw up 1mL vinegar 6. Remove needle from the syringe and discard 7. Attach 0.2-0.22um Whatman sterile syringe filter (do not touch the free end that will have a needle on it)
For Research Use Only 4
8. Put a new, sterile needle (~20 gauge) onto the free end of the Whatman filter (do not touch needle) a. Do not use the same needle on the Whatman that was used to originally draw up the unsterile vinegar and distilled water. 9. Put a ~20 gauge sterile needle into the top of your sterile glass vial to act as a vent 10. Inject the acetic acid solution into the vial 11. You are now done and should have sterile 0.6% acetic acid
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