Synthesis Of Testosterone

Posted: 04-Jun-2001 02:52 PM

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Excerpt: Here is some info I found on making testosterone…. Does anyone know if this will work? Materials needed-- Androstenedione (powder) Methanol Sodium Borohydride Acetic Acid Distilled Water Litmus Paper Thermometer Also: Beaker or glass container for the reaction, a pot for salt ice water bath, a spoon, fliter, an eropper (1 to 2c...

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Synthesis Of Testosterone

 

 

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Old 04-Jun-2001, 02:52 PM   #1

Here is some info I found on making testosterone….
Does anyone know if this will work?




Materials needed--

Androstenedione (powder)
Methanol
Sodium Borohydride
Acetic Acid
Distilled Water
Litmus Paper
Thermometer
Also:
Beaker or glass container for the reaction, a pot for salt ice water bath, a spoon, fliter, an eropper (1 to 2cc).

Procedure--

10 grams of Androstenedione is dissolved in 400ml methanol and cooled to 32 degrees Fahrenheit in a salt/ice bath (like making ice cream)

2.5g sodium borohydride is added while the solution is stirred.

Stir for 45 minutes while the temp is 32 degrees.

After 45 minutes, acetic acid is added, while stirring, in increments of 2ml at a time (hydrogen gas will evolve)

After each addition, the pH of the solution should be checked.

When the pH begins to turn acidic on the litmus paper, then stop.

Concentrate methanol solution to 50ml by evaporation.

Mix with 700ml distilled water.

Filter off the cloudy precipitate.

Wash filter cake with water several times.

Air or oven dry at a temp of no more that 150 degrees.

Final product will be 80% test, with the rest being unreacted andro and a mix of 3-alpha, 17-beta, and 3-beta, 17-beta androstendiols. All of which are also anabolic.

For administration, mix with propyleneglycol and or ethanol in a concentration of 30mg/ml, and take 1/3 of a millimeter, or 33insulin IUs under the tongue, one hour before training.
__________________________________________________ _________________
*Or this?*

SYNTHESIS OF testosterone FROM DHEA
Testerone from bacteria culture
US Patent # 2,236,574

Suspend 40g yeast in 160 ml water and add, if possible, 1.5g of disodium hydrogen phosphate and potassium dihydrogen phosphate. Shake in oxygen atmosphere for 1 day and then add lg (1000mg) DHEA suspended in 150ml of
water and shake for another 2 days in oxygen atmosphere. Then add 130g invert sugar (or brown sugar or honey) and let stand 3 days at room temperature. The mixture is then extracted with three 50ml portions of ether (shaken with the
ether then the ether separated) or methylene chloride, and the ether washed by shaking with water and separating ether solution. Evaporate ether to get testosterone.

SYNTHESIS OF ESTER OF testosterone
(cypionate and propionate)

Dissolve 5g testosterone (or analog) in 25mi pyridine or dry ether and add 3g propionic anhydride (or propionyl chloride) OR cypionyl chloride(cyclopentylpropionyl chloride) to get the propionate and cypionate
respectively. Let stand over night and add excess water, filter, wash, and dry.


Steroids Related to testosterone

Oil-soluble derivatives of testosterone itself predate those of its 19-nor congener, these agents too are used to administer depot injections so as to provide in effect long term blood levels of drug. Thus, acylation of testosterone with propionyl chloride in the presence of pyridine yields
testosterone propionate; acylation by means of decanoic anhydride yields testosterone decanoate. Finally, reaction with 3-cyclopentylpropionyl chloride affords testosterone cypionate. This last undergoes hydrolysis unusually slowly because of the presence of two substituents at the 6 postion.

Reaction of dehydroepiandrosterone (DHEA) with an excess of methylmagnesium bromide affords the 17a-methyl compound; again the aforementioned stedc effects lead to high stereoselectivity. Oppenauer oxidation of the resultant
intermediate proceeds with a shift of the double bond into conjugation to yield methyltestosterone. When the initial condensation is canted out with the Grignard reagent from allyl bromide instead, this sequence yields allylestrenol. Perhaps most startling is the fact that the product obtained
from the use of a metal acetylide in this synthesis . ethisterone, shows little if any, if any androgenic potency. Instead, the compound is an orally effective progestin.

These agents have all been used at one time as orally active
anabolic-androgenic agents. Dehydrogenation of methyltestosterone by means of chloranil extends the conjugation to afford the 4,6-diene-3-one- system. This
compound in turn undergoes 1,6 conjugate addition of methylmagnesium bromide in the presence of cuprous chloride to afford largely the 6a-methyl product,known as bolasterones.
Dehydrogenation with selenium dioxide, on the other hand, affords the cross conjugated diene, aka Dianabol.

Hydroxylation of the double bond of methyltestosterone by means of osmium tetro dde and hydrogen peroxide afford the 4,5 diol. This undergoes beta elimination on treatment with base to yield oxymestrone.

Catalytic reduction of DHEA goes as expected largely from the unhindered side of the molecule to afford a trans A/B dng fusion. Reaction with methyl Grignard reagent followed by oxidation of the intermediate yields androstanolone (1). Formylation of (1) with ethyl formate and base gives
oxymetholone (aka Anadrol), Catalytic reduction of the analogous hydroxmethylene compound from dihydrotestosterone propionate gives first the 28-methyl product. Treatment with base leads this to isomerize to the thermodynamically favored equatedal 2a-methyl compound., dromostanolone
propionate. The formyl ketone undergoes a reaction typical of this fuctional array on treatment with hydazine, leading to formation of the anabolic steroidal pyrazole, stanazole.

Last edited by randall_flagg; 04-Jun-2001 at 03:03 PM.
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wow that's phat

Old 04-Jun-2001, 04:47 PM   #2

Why don't you try it out and tell us how it goes
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Old 04-Jun-2001, 04:55 PM   #3

b_u_m_p
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Old 04-Jun-2001, 04:58 PM   #4

Good luck getting the materials. By the way, I suppose you are not a chemist, correct?
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Old 04-Jun-2001, 05:07 PM   #5

I am far from being a chemist, though I wish I were sometimes. Wouldn't it be nice to be able to wip up your own batch of test!
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Old 05-Jun-2001, 09:55 AM   #6

I have a friend that is a chemist. We went to school together. He lives in San Diego and works in TJ. I asked how tough it is to make Dianabol - methandrostenolone - , and he replied that it is not difficult, but large batches would be needed to get the economies of scale. He would not make me any and will not export any goodies over the border. His car is never searched.
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Old 05-Jun-2001, 10:07 AM   #7

Hmmm!!!!

Interesting! Nice post!


I hope someone can verify these methods!
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Re: Synthesis Of Testosterone

Old 06-May-2007, 07:42 PM   #8

where did you find that abstract for those synthesis steps?

Last edited by chem_kid; 07-May-2007 at 01:31 AM. Reason: no good
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Re: Synthesis Of Testosterone

Old 24-Dec-2012, 10:12 AM   #9

Anybody know how to convert testogel into an injectable form?
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Re: Synthesis Of Testosterone

Old 24-Dec-2012, 10:22 AM   #10

I am not a chemist as you must already have guessed, but as I understand it testosterone needs to be dissolved in an oil base to provide an intramuscular solution for injection. Is there any way to convert the gel (testogel) into a oil dissolved solution.
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